論文 - 詳細
| RRC ID | 46995 |
|---|---|
| 著者 | Saeki Y, Kudo T, Sone T, Kikuchi Y, Yokosawa H, Toh-e A, Tanaka K. |
| タイトル | Lysine 63-linked polyubiquitin chain may serve as a targeting signal for the 26S proteasome. |
| ジャーナル | EMBO J |
| Abstract |
Recruitment of substrates to the 26S proteasome usually requires covalent attachment of the Lys48-linked polyubiquitin chain. In contrast, modifications with the Lys63-linked polyubiquitin chain and/or monomeric ubiquitin are generally thought to function in proteasome-independent cellular processes. Nevertheless, the ubiquitin chain-type specificity for the proteasomal targeting is still poorly understood, especially in vivo. Using mass spectrometry, we found that Rsp5, a ubiquitin-ligase in budding yeast, catalyzes the formation of Lys63-linked ubiquitin chains in vitro. Interestingly, the 26S proteasome degraded well the Lys63-linked ubiquitinated substrate in vitro. To examine whether Lys63-linked ubiquitination serves in degradation in vivo, we investigated the ubiquitination of Mga2-p120, a substrate of Rsp5. The polyubiquitinated p120 contained relatively high levels of Lys63-linkages, and the Lys63-linked chains were sufficient for the proteasome-binding and subsequent p120-processing. In addition, Lys63-linked chains as well as Lys48-linked chains were detected in the 26S proteasome-bound polyubiquitinated proteins. These results raise the possibility that Lys63-linked ubiquitin chain also serves as a targeting signal for the 26S proteaseome in vivo. |
| 巻・号 | 28(4) |
| ページ | 359-71 |
| 公開日 | 2009-2-18 |
| DOI | 10.1038/emboj.2008.305 |
| PII | emboj2008305 |
| PMID | 19153599 |
| PMC | PMC2646160 |
| MeSH | Cyclin-Dependent Kinase Inhibitor Proteins Endosomal Sorting Complexes Required for Transport Lysine / chemistry* Mass Spectrometry / methods Membrane Proteins Models, Biological Plasmids / metabolism Polyubiquitin / chemistry* Proteasome Endopeptidase Complex / metabolism* Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / metabolism* Signal Transduction Trans-Activators / metabolism Transcription Factors Ubiquitin / chemistry Ubiquitin-Protein Ligase Complexes / metabolism* p120 GTPase Activating Protein / metabolism |
| IF | 9.889 |
| 引用数 | 155 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原微生物 | |