論文 - 詳細
| RRC ID | 47014 |
|---|---|
| 著者 | Oarada M, Kamei K, Gonoi T, Tsuzuki T, Toyotome T, Hirasaka K, Nikawa T, Sato A, Kurita N. |
| タイトル | Beneficial effects of a low-protein diet on host resistance to Paracoccidioides brasiliensis in mice. |
| ジャーナル | Nutrition |
| Abstract |
OBJECTIVE:Although protein malnutrition impairs immune functions, several studies have recently shown that protein restriction without malnutrition is beneficial to host defenses against invading pathogens and cancer. In an effort to establish the optimum diet for host resistance, we investigated the effect of different dietary protein levels on host resistance to Paracoccidioides brasiliensis. METHODS:Mice were fasted for 2 days and then infected with P. brasiliensis. Immediately after challenge with this fungus, mice were refed on diets with three different levels (0%, 1.5%, or 20%) of casein. On days 0-7 after infection, antifungal activity and levels of proinflammatory mediators in the spleen and liver were measured. RESULTS:Mice refed on the 1.5% casein diet showed higher antifungal activity in the spleen and liver compared with mice on the 20% casein diet. The antifungal activity in the spleens of mice refed on the 0% casein diet was intermediate between the antifungal activities of those refed the 1.5% and 20% casein diets. After infection, increases in spleen and liver levels of interleukin-6 and interferon-gamma, liver mRNA levels of antimicrobial proteins (myeloperoxidase, cathepsin-G, and elastase-2), and liver mRNA levels of proinflammatory mediators (interleukin-18, chemokine C-X-C motif ligand 10, nuclear factor-kappaB, inducible nitric oxide synthase, and granulocyte-macrophage colony-stimulating factor) were less profound in mice on the 1.5% or 0% casein diet compared with mice refed the 20% casein diet. CONCLUSION:The present results suggest that protein restriction without malnutrition could be beneficial to host resistance to P. brasiliensis. |
| 巻・号 | 25(9) |
| ページ | 954-63 |
| 公開日 | 2009-9-1 |
| DOI | 10.1016/j.nut.2009.02.004 |
| PII | S0899-9007(09)00088-4 |
| PMID | 19403266 |
| MeSH | Animals Caseins / pharmacology* Cathepsin G Cathepsins / metabolism Diet, Protein-Restricted* Dose-Response Relationship, Drug Female Gene Expression Immunity, Innate* Interferon-gamma / metabolism Interleukin-6 / metabolism Liver / drug effects Liver / immunology Liver / metabolism Mice Mice, Inbred BALB C Paracoccidioides* Paracoccidioidomycosis / diet therapy* Paracoccidioidomycosis / immunology Peroxidase / metabolism RNA, Messenger / metabolism Serine Endopeptidases / metabolism Spleen / drug effects Spleen / immunology Spleen / metabolism |
| IF | 3.639 |
| 引用数 | 14 |
| WOS 分野 | NUTRITION & DIETETICS |
| リソース情報 | |
| 病原微生物 | NA |