RRC ID 47086
著者 Nakazawa S, Shirae-Kurabayashi M, Otsuka K, Sawada H.
タイトル Proteomics of ionomycin-induced ascidian sperm reaction: Released and exposed sperm proteins in the ascidian Ciona intestinalis.
ジャーナル Proteomics
Abstract Sperm proteins mediating sperm-egg interaction should be exhibited on the sperm surface, or exposed or released when sperm approach an egg. In ascidians (protochordates), sperm undergo a sperm reaction, characterized by enhanced sperm motility and mitochondrial swelling and shedding on contact with the vitelline coat (VC) or by treatment with Ca(2+) ionophore. Here, proteomic analysis was conducted on sperm exudates and sperm surface proteins using ionomycin-induced sperm reaction and cell-impermeable labeling in Ciona intestinalis type A (C. robusta). In the exudate from sperm treated with ionomycin, membrane proteins including a possible VC receptor CiUrabin were abundant, indicating the release of membranous compartments during sperm reaction. Among the surface proteins XP_009859314.1 (uncharacterized protein exhibiting homology to HrTTSP-1) was most abundant before the sperm reaction, but XP_004227079.1 (unknown Ig superfamily protein) appears to be most abundantly exposed by the sperm reaction. Moreover, proteins containing a notable set of domains, astacin-like metalloprotease domain and thrombospondin type 1 repeat(s), were found in this fraction. Possible roles in fertilization as well as localizations and behaviors of these proteins are discussed.
巻・号 15(23-24)
ページ 4064-79
公開日 2015-8-1
DOI 10.1002/pmic.201500162
PMID 26223815
MeSH Animals Calcium Ionophores / pharmacology Ciona intestinalis / drug effects Ciona intestinalis / metabolism* Ionomycin / pharmacology* Male Proteasome Endopeptidase Complex / metabolism Proteomics* Spermatozoa / drug effects Spermatozoa / metabolism* Ubiquitin / metabolism
IF 4.041
引用数 1
WOS 分野 BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
カタユウレイボヤ・(ニッポンウミシダ) Wild C. int