論文 - 詳細
| RRC ID | 47086 |
|---|---|
| 著者 | Nakazawa S, Shirae-Kurabayashi M, Otsuka K, Sawada H. |
| タイトル | Proteomics of ionomycin-induced ascidian sperm reaction: Released and exposed sperm proteins in the ascidian Ciona intestinalis. |
| ジャーナル | Proteomics |
| Abstract |
Sperm proteins mediating sperm-egg interaction should be exhibited on the sperm surface, or exposed or released when sperm approach an egg. In ascidians (protochordates), sperm undergo a sperm reaction, characterized by enhanced sperm motility and mitochondrial swelling and shedding on contact with the vitelline coat (VC) or by treatment with Ca(2+) ionophore. Here, proteomic analysis was conducted on sperm exudates and sperm surface proteins using ionomycin-induced sperm reaction and cell-impermeable labeling in Ciona intestinalis type A (C. robusta). In the exudate from sperm treated with ionomycin, membrane proteins including a possible VC receptor CiUrabin were abundant, indicating the release of membranous compartments during sperm reaction. Among the surface proteins XP_009859314.1 (uncharacterized protein exhibiting homology to HrTTSP-1) was most abundant before the sperm reaction, but XP_004227079.1 (unknown Ig superfamily protein) appears to be most abundantly exposed by the sperm reaction. Moreover, proteins containing a notable set of domains, astacin-like metalloprotease domain and thrombospondin type 1 repeat(s), were found in this fraction. Possible roles in fertilization as well as localizations and behaviors of these proteins are discussed. |
| 巻・号 | 15(23-24) |
| ページ | 4064-79 |
| 公開日 | 2015-12-1 |
| DOI | 10.1002/pmic.201500162 |
| PMID | 26223815 |
| MeSH | Animals Calcium Ionophores / pharmacology Ciona intestinalis / drug effects Ciona intestinalis / metabolism* Ionomycin / pharmacology* Male Proteasome Endopeptidase Complex / metabolism Proteomics* Spermatozoa / drug effects Spermatozoa / metabolism* Ubiquitin / metabolism |
| IF | 3.254 |
| 引用数 | 6 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カタユウレイボヤ・(ニッポンウミシダ) | Wild C. int |