| RRC ID |
4709
|
| 著者 |
Fujii T, Abe H, Katsuma S, Mita K, Shimada T.
|
| タイトル |
Mapping of sex-linked genes onto the genome sequence using various aberrations of the Z chromosome in Bombyx mori.
|
| ジャーナル |
Insect Biochem Mol Biol
|
| Abstract |
Many strains of Bombyx mori carry chromosomal aberrations, and they are useful resources for integration between phenotypes and genomic sequences. We compared the molecular structures of three kinds of Z chromosomes, i.e., two strains with chromosome deletions and one strain with translocation involving the Z chromosome. Using polymerase chain reaction markers, we showed that: (1) the Z(1) chromosome lacks more than 6Mb, including the proximal end; (2) the Z(Vg) chromosome lacks 1.5Mb in the interstitial portion; and (3) the +(od)p(Sa)+(p)W carries a 0.6-Mb Z-derived fragment surrounding the +(od) gene. The breakpoint junctions of these deletions and a translocation were precisely determined. Through deletion mapping, we narrowed down the regions where distinct oily (od), vestigial (Vg), and muscle dystrophy (Md) are located and identified a candidate gene for od. A retroposon-mediated deletion in BmBLOS2--the Bombyx gene homologous to human "biogenesis of lysosome-related organelles complex-1, subunit 2''--was detected in the od mutant. Although the genes responsible for Vg and Md were not definitively identified, we propose the candidate genes on the basis of their locations and phenotypes.
|
| 巻・号 |
38(12)
|
| ページ |
1072-9
|
| 公開日 |
2008-12-1
|
| DOI |
10.1016/j.ibmb.2008.03.004
|
| PII |
S0965-1748(08)00044-1
|
| 解説 |
BmBlos2はヒトのヘルマンスキーパドラック症候群の原因遺伝子の一つであるBlos2のカイコホモログである。od系統では、BmBlos2のエクソン１～３が欠失している。この欠失には、BmBlos2のエクソン１の上流とイントロン３に共通して存在するトランスポゾンBm1の関与が推定されている。
Vg系統(u01)のZ染色体には、1.8 Mbの中間部欠失が存在する。この欠失が原因で、通常は1.8 Mb離れている二つの遺伝子(Bmptp-ZとBmap-A)から融合転写産物が生じる (Fujii et al., 2011)。
|
| PMID |
19216995
|
| MeSH |
Animals
Bombyx / genetics*
Chromosome Mapping / methods*
Female
Genome, Insect*
Male
Sex Chromosome Aberrations*
Sex Chromosomes*
|
| IF |
3.827
|
| 引用数 |
27
|
|
WOS 分野
|
ENTOMOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
u01 |
