RRC ID 47214
著者 Yoshida T, Sakamoto T.
タイトル Water-stress induced trehalose accumulation and control of trehalase in the cyanobacterium Nostoc punctiforme IAM M-15.
ジャーナル J Gen Appl Microbiol
Abstract We investigated the biochemical properties of the enzymes involved in trehalose metabolism in the cyanobacterium Nostoc punctiforme strain IAM M-15 to elucidate the mechanism of trehalose accumulation in response to desiccation and salt stress. There was no detectable trehalose in fully hydrated N. punctiforme cells; however, these cells accumulated trehalose upon desiccation. Moreover, NaCl treatment also induced trehalose accumulation. The three genes for trehalose metabolism, treZ (maltooligosyltrehalose trehalohydrolase, Mth), treY (maltooligosyltrehalose synthase, Mts), and treH (trehalase), were found as a gene cluster, and the mRNAs for these genes were detectable at similar levels during desiccation. Trehalase of N. punctiforme was heterologously expressed in E. coli cells in an active form with a molecular mass of 52 kDa. Trehalase activity was strongly inhibited in the presence of 10 mM NaCl while trehalose synthesis activity remained active in the presence of salt. These data suggest that the rate of trehalose production exceeds that of trehalose hydrolysis under water-stress conditions characterized by increased cellular solute concentrations. In the proposed mechanism, control of trehalase plays an important role in trehalose accumulation in terrestrial cyanobacteria under conditions of extreme desiccation.
巻・号 55(2)
ページ 135-45
公開日 2009-4-1
DOI 10.2323/jgam.55.135
PII JST.JSTAGE/jgam/55.135
PMID 19436130
MeSH Dehydration / enzymology Dehydration / genetics Gene Expression Regulation, Bacterial / drug effects Gene Expression Regulation, Bacterial / physiology* Genes, Bacterial / genetics Nostoc / drug effects Nostoc / enzymology* Nostoc / metabolism RNA, Messenger / metabolism Recombinant Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Sodium Chloride / pharmacology Stress, Physiological / physiology Sucrose / metabolism Trehalase / genetics Trehalase / metabolism* Trehalose / genetics Trehalose / metabolism*
IF 1.442
引用数 26
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
藻類 NIES-2108