Reference - Detail
| RRC ID | 47271 |
|---|---|
| Author | Buhl E, Bradlaugh A, Ogueta M, Chen KF, Stanewsky R, Hodge JJ. |
| Title | Quasimodo mediates daily and acute light effects on Drosophila clock neuron excitability. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
We have characterized a light-input pathway regulating Drosophila clock neuron excitability. The molecular clock drives rhythmic electrical excitability of clock neurons, and we show that the recently discovered light-input factor Quasimodo (Qsm) regulates this variation, presumably via an Na+, K+, Cl- cotransporter (NKCC) and the Shaw K+ channel (dKV3.1). Because of light-dependent degradation of the clock protein Timeless (Tim), constant illumination (LL) leads to a breakdown of molecular and behavioral rhythms. Both overexpression (OX) and knockdown (RNAi) of qsm, NKCC, or Shaw led to robust LL rhythmicity. Whole-cell recordings of the large ventral lateral neurons (l-LNv) showed that altering Qsm levels reduced the daily variation in neuronal activity: qsmOX led to a constitutive less active, night-like state, and qsmRNAi led to a more active, day-like state. Qsm also affected daily changes in K+ currents and the GABA reversal potential, suggesting a role in modifying membrane currents and GABA responses in a daily fashion, potentially modulating light arousal and input to the clock. When directly challenged with blue light, wild-type l-LNvs responded with increased firing at night and no net response during the day, whereas altering Qsm, NKKC, or Shaw levels abolished these day/night differences. Finally, coexpression of ShawOX and NKCCRNAi in a qsm mutant background restored LL-induced behavioral arrhythmicity and wild-type neuronal activity patterns, suggesting that the three genes operate in the same pathway. We propose that Qsm affects both daily and acute light effects in l-LNvs probably acting on Shaw and NKCC. |
| Volume | 113(47) |
| Pages | 13486-13491 |
| Published | 2016-11-22 |
| DOI | 10.1073/pnas.1606547113 |
| PII | 1606547113 |
| PMID | 27821737 |
| PMC | PMC5127355 |
| MeSH | Alleles Animals Behavior, Animal Circadian Clocks / radiation effects* Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / physiology* Drosophila melanogaster / radiation effects* GPI-Linked Proteins / metabolism* Gene Knockdown Techniques Genotype Ion Channel Gating / radiation effects Light* Models, Biological Neurons / physiology* Neurons / radiation effects* Protein Binding / radiation effects gamma-Aminobutyric Acid / metabolism |
| IF | 9.412 |
| Times Cited | 12 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 13 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | 2509R-2 |