論文 - 詳細
| RRC ID | 47285 |
|---|---|
| 著者 | Terajima H, Yoshitane H, Ozaki H, Suzuki Y, Shimba S, Kuroda S, Iwasaki W, Fukada Y. |
| タイトル | ADARB1 catalyzes circadian A-to-I editing and regulates RNA rhythm. |
| ジャーナル | Nat Genet |
| Abstract |
It has been proposed that the CLOCK-ARNTL (BMAL1) complex drives circadian transcription of thousands of genes, including Per and Cry family genes that encode suppressors of CLOCK-ARNTL-dependent transcription. However, recent studies demonstrated that 70-80% of circadian-oscillating mRNAs have no obvious rhythms in their de novo transcription, indicating the potential importance of post-transcriptional regulation. Our CLOCK-ChIP-seq analysis identified rhythmic expression of adenosine deaminase, RNA-specific, B1 (Adarb1, also known as Adar2), an adenosine-to-inosine (A-to-I) RNA-editing enzyme. RNA-seq showed circadian rhythms of ADARB1-mediated A-to-I editing in a variety of transcripts. In Adarb1-knockout mice, rhythms of large populations of mRNA were attenuated, indicating a profound impact of ADARB1-mediated A-to-I editing on RNA rhythms. Furthermore, Adarb1-knockout mice exhibited short-period rhythms in locomotor activity and gene expression. These phenotypes were associated with abnormal accumulation of CRY2. The present study identifies A-to-I RNA editing as a key mechanism of post-transcriptional regulation in the circadian clockwork. |
| 巻・号 | 49(1) |
| ページ | 146-151 |
| 公開日 | 2017-1-1 |
| DOI | 10.1038/ng.3731 |
| PII | ng.3731 |
| PMID | 27893733 |
| MeSH | Adenosine / genetics* Adenosine Deaminase / genetics Adenosine Deaminase / metabolism* Animals Aryl Hydrocarbon Receptor Nuclear Translocator / physiology Circadian Rhythm / physiology* Cryptochromes / genetics Cryptochromes / metabolism Gene Expression Regulation Humans Inosine / genetics* Liver / metabolism Male Mice Mice, Inbred C57BL Mice, Knockout RNA / genetics* RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Transcription, Genetic / genetics* |
| IF | 27.605 |
| 引用数 | 32 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 34 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NIH3T3-3-4(RCB1862) |