RRC ID 47324
Author Morita T, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Nagahama H, Oka S, Oshigiri T, Takebayashi T, Yamashita T, Kocsis JD, Honmou O.
Title Intravenous infusion of mesenchymal stem cells promotes functional recovery in a model of chronic spinal cord injury.
Journal Neuroscience
Abstract Intravenous infusion of mesenchymal stem cells (MSCs) derived from adult bone marrow improves behavioral function in rat models of spinal cord injury (SCI). However, most studies have focused on the acute or subacute phase of SCI. In the present study, MSCs derived from bone marrow of rats were intravenously infused 10weeks after the induction of a severe contusive SCI. Open field locomotor function was assessed weekly until 20weeks post-SCI. Motor recovery was greater in the MSC-treated group with rapid improvement beginning in earlier post-infusion times than in the vehicle-treated group. Blood spinal cord barrier (BSCB) integrity was assessed by the intravenous infusion of Evans Blue (EvB) with spectrophotometric quantitation of its leakage into the parenchyma. In MSC-treated rats, BSCB leakage was reduced. Immunohistochemical staining for RECA-1 and PDGFR-β showed increased microvasculature/repair-neovascularization in MSC-treated rats. There was extensive remyelination around the lesion center and increased sprouting of the corticospinal tract and serotonergic fibers after MSC infusion. These results indicate that the systemic infusion of MSCs results in functional improvement that is associated with structural changes in the chronically injured spinal cord including stabilization of the BSCB, axonal sprouting/regeneration and remyelination.
Volume 335
Pages 221-31
Published 2016-10-29
DOI 10.1016/j.neuroscience.2016.08.037
PII S0306-4522(16)30410-9
PMID 27586052
MeSH Animals Bone Marrow Cells / cytology Bone Marrow Transplantation / methods Chronic Disease Disease Models, Animal Infusions, Intravenous / methods Mesenchymal Stem Cells / cytology* Motor Activity / physiology* Nerve Regeneration / physiology Rats, Sprague-Dawley Recovery of Function / physiology* Spinal Cord / pathology Spinal Cord Injuries / pathology* Spinal Cord Injuries / physiopathology
IF 3.244
Times Cited 49
Rats W-Tg (CAG-GFP) 184Ys (strainID=525)