RRC ID 47448
Author Low VF, Mombaerts P.
Title Odorant receptor proteins in the mouse main olfactory epithelium and olfactory bulb.
Journal Neuroscience
Abstract In the mouse, odorant receptor proteins (ORs) are G-protein-coupled receptors expressed in mature olfactory sensory neurons (OSNs) of the main olfactory epithelium (MOE). ORs mediate odorant reception at the level of the OSN cilia. Most of the ∼1100 OR genes in the mouse genome are expressed, at the RNA level, in mature OSNs. The literature on antibodies against ORs is limited, and most reports are with antibodies that are not commercially available. Here we have screened 40 commercial antibodies against human and mouse ORs by immunofluorescence staining of coronal cryosections of the MOE of 21-day-old C57BL/6J mice. Various methods of antigen retrieval were tested. Of the 19 antibodies raised against human ORs, three yielded a consistent immunoreactive signal in the mouse MOE; of these three, two appeared to cross react against one or more, unknown, mouse ORs. Of the 21 antibodies raised against mouse ORs, six yielded a consistent immunoreactive signal in the mouse MOE; of these six, two also stained specific glomeruli in the olfactory bulb. Antibody specificity could be validated with gene-targeted mouse strains in the case of three ORs. The number of OSNs immunoreactive for the MOR28/Olfr1507 antibody is greater in C57BL/6J than in 129S6/SvEvTac wild-type mice. Taken together, our results are encouraging: 20-30% of these commercially available antibodies are informative in immunohistochemical analyses of the mouse MOE. The commercial availability of these antibodies should facilitate the study of OR proteins in the MOE and the olfactory bulb, and the replicability of results in the literature.
Volume 344
Pages 167-177
Published 2017-3-6
DOI 10.1016/j.neuroscience.2016.12.044
PII S0306-4522(16)30744-8
PMID 28057532
MeSH Animals Antibodies / metabolism Cell Count Fluorescent Antibody Technique Humans In Situ Hybridization Mice Mice, 129 Strain Mice, Inbred C57BL Mice, Transgenic Microscopy, Confocal Olfactory Bulb / cytology Olfactory Bulb / metabolism* Olfactory Mucosa / cytology Olfactory Mucosa / metabolism* Receptors, Odorant / genetics Receptors, Odorant / metabolism* Species Specificity
IF 3.056
Times Cited 4
Mice RBRC02928