論文 - 詳細
| RRC ID | 47456 |
|---|---|
| 著者 | Funabashi T, Katoh Y, Michisaka S, Terada M, Sugawa M, Nakayama K. |
| タイトル | Ciliary entry of KIF17 is dependent on its binding to the IFT-B complex via IFT46-IFT56 as well as on its nuclear localization signal. |
| ジャーナル | Mol Biol Cell |
| Abstract |
Cilia function as cellular antennae to sense and transduce extracellular signals. A number of proteins are specifically localized in cilia. Anterograde and retrograde ciliary protein trafficking are mediated by the IFT-B and IFT-A complexes in concert with kinesin-2 and dynein-2 motors, respectively. However, the role of KIF17, a homodimeric kinesin-2 protein, in protein trafficking has not been fully understood in vertebrate cilia. In this study, we demonstrated, by using the visible immunoprecipitation assay, that KIF17 interacts with the IFT46-IFT56 dimer in the IFT-B complex through its C-terminal sequence located immediately upstream of the nuclear localization signal (NLS). We then showed that KIF17 reaches the ciliary tip independently of its motor domain and requires IFT-B binding for its entry into cilia rather than for its intraciliary trafficking. We further showed that KIF17 ciliary entry depends not only on its binding to IFT-B but also on its NLS, to which importin α proteins bind. Taking the results together, we conclude that in mammalian cells, KIF17 is dispensable for ciliogenesis and IFT-B trafficking but requires IFT-B, as well as its NLS, for its ciliary entry across the permeability barrier located at the ciliary base. |
| 巻・号 | 28(5) |
| ページ | 624-633 |
| 公開日 | 2017-3-1 |
| DOI | 10.1091/mbc.E16-09-0648 |
| PII | mbc.E16-09-0648 |
| PMID | 28077622 |
| PMC | PMC5328621 |
| MeSH | Axoneme / metabolism Carrier Proteins / metabolism* Cell Line Cilia / metabolism* Dyneins / metabolism Flagella / metabolism Gene Knockout Techniques Humans Kinesins / metabolism* Molecular Motor Proteins / metabolism* Nuclear Localization Signals / metabolism Protein Transport / physiology Retinal Pigment Epithelium / cytology Retinal Pigment Epithelium / metabolism |
| IF | 3.791 |
| 引用数 | 23 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | Genome Network Project human cDNA clone IRAK140P09 (HGX056369) pCAG-EGFP-C-KIF17 (RDB15196) pCAG-mCherry-C-KIF17 (RDB15197). |