RRC ID 47473
Author Wen J, Brogna S.
Title Splicing-dependent NMD does not require the EJC in Schizosaccharomyces pombe.
Journal EMBO J.
Abstract Nonsense-mediated mRNA decay (NMD) is a translation-linked process that destroys mRNAs with premature translation termination codons (PTCs). In mammalian cells, NMD is also linked to pre-mRNA splicing, usually PTCs trigger strong NMD only when positioned upstream of at least one intron. The exon junction complex (EJC) is believed to mediate the link between splicing and NMD in these systems. Here, we report that in Schizosaccharomyces pombe splicing also enhances NMD, but against the EJC model prediction, an intron stimulated NMD regardless of whether it is positioned upstream or downstream of the PTC and EJC components are not required. Still the effect of splicing seems to be direct-we have found that the important NMD determinant is the proximity of an intron to the PTC, not just the occurrence of splicing. On the basis of these results, we propose a new model to explain how splicing could affect NMD.
Volume 29(9)
Pages 1537-51
Published 2010-5-5
DOI 10.1038/emboj.2010.48
PII emboj201048
PMID 20360683
PMC PMC2876954
MeSH 3' Untranslated Regions Codon / genetics Exons* Introns Protein Biosynthesis RNA Splicing* RNA, Bacterial / genetics* RNA, Messenger / genetics* Schizosaccharomyces / genetics*
IF 10.557
Times Cited 33
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
DNA material pDUAL (RDB06178)