RRC ID 47488
Author Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, Verkhusha VV.
Title Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging.
Journal Nat Commun
Abstract Monomeric near-infrared (NIR) fluorescent proteins (FPs) are in high demand as protein tags and components of biosensors for deep-tissue imaging and multicolour microscopy. We report three bright and spectrally distinct monomeric NIR FPs, termed miRFPs, engineered from bacterial phytochrome, which can be used as easily as GFP-like FPs. miRFPs are 2-5-fold brighter in mammalian cells than other monomeric NIR FPs and perform well in protein fusions, allowing multicolour structured illumination microscopy. miRFPs enable development of several types of NIR biosensors, such as for protein-protein interactions, RNA detection, signalling cascades and cell fate. We demonstrate this by engineering the monomeric fluorescence complementation reporters, the IκBα reporter for NF-κB pathway and the cell cycle biosensor for detection of proliferation status of cells in culture and in animals. miRFPs allow non-invasive visualization and detection of biological processes at different scales, from super-resolution microscopy to in vivo imaging, using the same probes.
Volume 7
Pages 12405
Published 2016-8-19
DOI 10.1038/ncomms12405
PII ncomms12405
PMID 27539380
PMC PMC4992171
MeSH Animals Bacteria / genetics Bacteria / metabolism* Bacterial Proteins / chemistry Bacterial Proteins / genetics* Biosensing Techniques / methods* Cell Line Female Hepatocytes Humans Infrared Rays Intravital Microscopy / methods* Luminescent Proteins / chemistry Luminescent Proteins / genetics* Mice Mice, SCID Mutagenesis Phytochrome / chemistry Phytochrome / genetics Protein Engineering Spectroscopy, Near-Infrared / methods
IF 12.353
Times Cited 7
DNA material CSII-EF-MCS (RDB04378)