論文 - 詳細
| RRC ID | 47488 |
|---|---|
| 著者 | Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, Verkhusha VV. |
| タイトル | Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging. |
| ジャーナル | Nat Commun |
| Abstract |
Monomeric near-infrared (NIR) fluorescent proteins (FPs) are in high demand as protein tags and components of biosensors for deep-tissue imaging and multicolour microscopy. We report three bright and spectrally distinct monomeric NIR FPs, termed miRFPs, engineered from bacterial phytochrome, which can be used as easily as GFP-like FPs. miRFPs are 2-5-fold brighter in mammalian cells than other monomeric NIR FPs and perform well in protein fusions, allowing multicolour structured illumination microscopy. miRFPs enable development of several types of NIR biosensors, such as for protein-protein interactions, RNA detection, signalling cascades and cell fate. We demonstrate this by engineering the monomeric fluorescence complementation reporters, the IκBα reporter for NF-κB pathway and the cell cycle biosensor for detection of proliferation status of cells in culture and in animals. miRFPs allow non-invasive visualization and detection of biological processes at different scales, from super-resolution microscopy to in vivo imaging, using the same probes. |
| 巻・号 | 7 |
| ページ | 12405 |
| 公開日 | 2016-8-19 |
| DOI | 10.1038/ncomms12405 |
| PII | ncomms12405 |
| PMID | 27539380 |
| PMC | PMC4992171 |
| MeSH | Animals Bacteria / genetics Bacteria / metabolism* Bacterial Proteins / chemistry Bacterial Proteins / genetics* Biosensing Techniques / methods* Cell Line Female Hepatocytes Humans Infrared Rays Intravital Microscopy / methods* Luminescent Proteins / chemistry Luminescent Proteins / genetics* Mice Mice, SCID Mutagenesis Phytochrome / chemistry Phytochrome / genetics Protein Engineering Spectroscopy, Near-Infrared / methods |
| IF | 12.121 |
| 引用数 | 89 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 25 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 遺伝子材料 | CSII-EF-MCS (RDB04378) |