RRC ID 475
Author Koike Y, Mita K, Suzuki MG, Maeda S, Abe H, Osoegawa K, deJong PJ, Shimada T.
Title Genomic sequence of a 320-kb segment of the Z chromosome of Bombyx mori containing a kettin ortholog.
Journal Mol. Genet. Genomics
Abstract The sex chromosome constitution of the silkworm, Bombyx mori, is ZW in the female and ZZ in the male. Very little molecular information is available about the Z chromosome in Lepidoptera, although the topic is interesting because of the absence of gene dosage compensation in this chromosome. We constructed a 320-kb BAC contig around the Bmkettin gene on the Z chromosome in Bombyx and determined its nucleotide sequence by the shotgun method. We found 13 novel protein-coding sequences in addition to Bmkettin. All the transposable elements detected in the region were truncated, and no LTR retrotransposons were found, in stark contrast to the situation on the W chromosome. In this 320-kb region, four genes for muscle proteins (Bmkettin, Bmtitin1, Bmtitin2, and Bmprojectin) are clustered, together with another gene (Bmmiple) on the Z chromosome in B. mori; their orthologs are also closely linked on chromosome 3 in Drosophila, suggesting a partial synteny. Real-time RT-PCR experiments demonstrated that transcripts of 13 genes of the 14 Z-linked genes found accumulated in larger amounts in males than in female moths, indicating the absence of gene dosage compensation. The implications of these findings for the evolution and function of the Z chromosome in Lepidoptera are discussed.
Volume 269(1)
Pages 137-49
Published 2003-4
DOI 10.1007/s00438-003-0822-6
PMID 12715162
MeSH Amino Acid Motifs Amino Acid Sequence Animals Base Sequence Bombyx / genetics* Chromosome Mapping Chromosome Walking Chromosomes, Artificial, Bacterial / genetics Connectin Contig Mapping Drosophila Proteins* Expressed Sequence Tags Female Gene Library Genes, Insect* Insect Proteins / genetics* Male Molecular Sequence Data Muscle Proteins / genetics* Sequence Analysis, DNA Sex Characteristics Sex Chromosomes / genetics*
IF 2.879
Times Cited 55
Silkworms p50 EST database