論文 - 詳細
| RRC ID | 47505 |
|---|---|
| 著者 | O'Connell Motherway M, Fitzgerald GF, van Sinderen D. |
| タイトル | Metabolism of a plant derived galactose-containing polysaccharide by Bifidobacterium breve UCC2003. |
| ジャーナル | Microb Biotechnol |
| Abstract |
In this study, we describe the functional characterization of the Bifidobacterium breve UCC2003 gal locus, which is dedicated to the utilization of galactan, a plant-derived polysaccharide. Using a combination of molecular approaches we conclude that the galA gene of B. breve UCC2003 encodes a β-1,4-endogalactanase producing galacto-oligosaccharides, which are specifically internalized by an ABC transport system, encoded by galBCDE, and which are then hydrolysed to galactose moieties by a dedicated intracellular β-galactosidase, specified by galG. The generated galactose molecules are presumed to be fed into the fructose-6-phosphate phosphoketolase pathway via the Leloir pathway, thereby allowing B. breve UCC2003 to use galactan as its sole carbon and energy source. In addition to these findings we demonstrate that GalR is a LacI-type DNA-binding protein, which not only appears to control transcription of the galCDEGR operon, but also that of the galA gene. |
| 巻・号 | 4(3) |
| ページ | 403-16 |
| 公開日 | 2011-5-1 |
| DOI | 10.1111/j.1751-7915.2010.00218.x |
| PMID | 21375716 |
| PMC | PMC3818998 |
| MeSH | Bacterial Proteins / genetics Bacterial Proteins / metabolism Bifidobacterium / enzymology Bifidobacterium / genetics Bifidobacterium / growth & development Bifidobacterium / metabolism* Galactans / analysis Galactans / metabolism* Galactose / analysis Galactose / metabolism* Gene Expression Regulation, Bacterial Glycoside Hydrolases / genetics Glycoside Hydrolases / metabolism Molecular Sequence Data Solanum tuberosum / chemistry Solanum tuberosum / metabolism* |
| IF | 5.328 |
| 引用数 | 60 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 7017 JCM 7019 JCM 20097 JCM 7050 JCM 7052 JCM 7053 JCM 7056 JCM 5820 JCM 7092 JCM 7027 |