RRC ID |
47509
|
Author |
Inoue R, Nagino T, Hoshino G, Ushida K.
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Title |
Nucleic acids of Enterococcus faecalis strain EC-12 are potent Toll-like receptor 7 and 9 ligands inducing interleukin-12 production from murine splenocytes and murine macrophage cell line J774.1.
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Journal |
FEMS Immunol Med Microbiol
|
Abstract |
In experiment 1 of this study, the interleukin-12 (IL-12)-inducing ability of six Enterococcus strains was evaluated in comparison with that of five Lactobacillus strains using murine splenocytes. At the same time, the involvement of Toll-like receptor (TLR) ligands in IL-12-inducing ability was assessed using splenocytes from TLR2-, TLR4- and MyD88-deficient mice. Most Enterococcus strains, especially Enterococcus faecalis strain EC-12, exerted higher IL-12-inducing ability compared with the Lactobacillus strains evaluated. Almost the same amount of IL-12 protein was produced by all lactic acid bacteria strains in splenocytes from TLR2- and TLR4-deficient mice, whereas splenocytes from MyD88-deficient mice showed no IL-12 production against all bacteria evaluated. In experiment 2, the role of TLR7, 8 and 9 ligands of E. faecalis strain EC-12 in the induction of IL-12 production was evaluated using murine macrophage cell line J774.1. A drastic decrease in IL-12-inducing ability was observed when heat-killed E. faecalis strain EC-12 was treated with nuclease, particularly RNase. In addition, less than one-tenth of IL-12 was produced by heat-killed E. faecalis strain EC-12 when both TLR7 and 9 were antagonized. These facts indicate that the nucleic acids of E. faecalis strain EC-12, particularly its RNA, are the potent TLR7 and 9 ligands that induce IL-12 production from antigen-presenting cells.
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Volume |
61(1)
|
Pages |
94-102
|
Published |
2011-2-1
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DOI |
10.1111/j.1574-695X.2010.00752.x
|
PMID |
21073545
|
MeSH |
Animals
Antigen-Presenting Cells / immunology
Cell Line
DNA, Bacterial / immunology
Enterococcus faecalis / genetics*
Interleukin-12 / immunology*
Ligands*
Mice
Mice, Inbred C57BL
Mice, Knockout
Myeloid Differentiation Factor 88 / immunology
Nucleic Acids / immunology*
RNA, Bacterial / immunology
Signal Transduction / immunology
Spleen / immunology*
Toll-Like Receptors / immunology*
|
Times Cited |
16
|
WOS Category
|
INFECTIOUS DISEASES
MICROBIOLOGY
IMMUNOLOGY
|
Resource |
General Microbes |
JCM 8714
JCM 8727
JCM 1132
JCM 1134
JCM 5344
JCM 1149 |