論文 - 詳細
| RRC ID | 47509 |
|---|---|
| 著者 | Inoue R, Nagino T, Hoshino G, Ushida K. |
| タイトル | Nucleic acids of Enterococcus faecalis strain EC-12 are potent Toll-like receptor 7 and 9 ligands inducing interleukin-12 production from murine splenocytes and murine macrophage cell line J774.1. |
| ジャーナル | FEMS Immunol Med Microbiol |
| Abstract |
In experiment 1 of this study, the interleukin-12 (IL-12)-inducing ability of six Enterococcus strains was evaluated in comparison with that of five Lactobacillus strains using murine splenocytes. At the same time, the involvement of Toll-like receptor (TLR) ligands in IL-12-inducing ability was assessed using splenocytes from TLR2-, TLR4- and MyD88-deficient mice. Most Enterococcus strains, especially Enterococcus faecalis strain EC-12, exerted higher IL-12-inducing ability compared with the Lactobacillus strains evaluated. Almost the same amount of IL-12 protein was produced by all lactic acid bacteria strains in splenocytes from TLR2- and TLR4-deficient mice, whereas splenocytes from MyD88-deficient mice showed no IL-12 production against all bacteria evaluated. In experiment 2, the role of TLR7, 8 and 9 ligands of E. faecalis strain EC-12 in the induction of IL-12 production was evaluated using murine macrophage cell line J774.1. A drastic decrease in IL-12-inducing ability was observed when heat-killed E. faecalis strain EC-12 was treated with nuclease, particularly RNase. In addition, less than one-tenth of IL-12 was produced by heat-killed E. faecalis strain EC-12 when both TLR7 and 9 were antagonized. These facts indicate that the nucleic acids of E. faecalis strain EC-12, particularly its RNA, are the potent TLR7 and 9 ligands that induce IL-12 production from antigen-presenting cells. |
| 巻・号 | 61(1) |
| ページ | 94-102 |
| 公開日 | 2011-2-1 |
| DOI | 10.1111/j.1574-695X.2010.00752.x |
| PMID | 21073545 |
| MeSH | Animals Antigen-Presenting Cells / immunology Cell Line DNA, Bacterial / immunology Enterococcus faecalis / genetics* Interleukin-12 / immunology* Ligands* Mice Mice, Inbred C57BL Mice, Knockout Myeloid Differentiation Factor 88 / immunology Nucleic Acids / immunology* RNA, Bacterial / immunology Signal Transduction / immunology Spleen / immunology* Toll-Like Receptors / immunology* |
| 引用数 | 16 |
| WOS 分野 | INFECTIOUS DISEASES MICROBIOLOGY IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 8714 JCM 8727 JCM 1132 JCM 1134 JCM 5344 JCM 1149 |