| RRC ID |
47514
|
| 著者 |
Abdel-Mohsein HS, Sasaki T, Tada C, Nakai Y.
|
| タイトル |
Characterization and partial purification of a bacteriocin-like substance produced by thermophilic Bacillus licheniformis H1 isolated from cow manure compost.
|
| ジャーナル |
Anim Sci J
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| Abstract |
The production and partial characterization of bacteriocin-like substances (BLSs) produced by bacteria isolated from cow manure compost were investigated. Eight BLS producers, which exhibited inhibitory activity against pathogenic bacteria, were isolated from cow manure compost at different stages of the composting process. The pile temperature ranged from 9.1 °C to 73.2 °C. The BLSs showed thermostability, but the BLS producers were not thermostable except for the H1 producer. Thermophilic Bacillus licheniformis H1 was further characterized. The culture supernatant of B. licheniformis H1 exhibited antagonistic activity against various species of Gram-positive bacteria such as Listeria monocytogenes ATCC19111 but not against Gram-negative bacteria except Pseudomonas fluorescens ATCC11251. Inactivation of bacteriocin-like activity by α-chymotrypsin, trypsin, and papain was highly significant (P<0.001). The BLS was found to be stable under a pH range from 3 to 9 and at temperatures up to 75 °C for 60 min, but it lost activity after being autoclaved at 121 °C for 15 min. The optimum production of BLS by B. licheniformis H1 was obtained at a temperature of 55 °C. Sodium dodecyl sulfate - polyacrylamide electrophoresis analysis of concentrated partially purified supernatants collected after resting the bacterial cells at 55 °C revealed a bacteriocin-like protein with a molecular mass of approximately 3.5 kDa. This study is the first report of a BLS from thermophilic B. licheniformis with an animal compost origin.
|
| 巻・号 |
82(2)
|
| ページ |
340-51
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| 公開日 |
2011-4-1
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| DOI |
10.1111/j.1740-0929.2010.00835.x
|
| PMID |
21729216
|
| MeSH |
Animals
Bacillus / isolation & purification
Bacillus / metabolism*
Bacteriocins / biosynthesis
Bacteriocins / isolation & purification*
Bacteriocins / pharmacology
Cattle
Gram-Negative Bacteria / drug effects
Gram-Positive Bacteria / drug effects
Hot Temperature
Manure / microbiology*
Soil Microbiology
|
| IF |
1.399
|
| 引用数 |
6
|
|
WOS 分野
|
AGRICULTURE, DAIRY & ANIMAL SCIENCE
|
| リソース情報 |
| 一般微生物 |
JCM 5803
JCM 2151
JCM 7577 |
