RRC ID 47524
Author Hara KY, Suzuki R, Suzuki T, Yoshida M, Kino K.
Title ATP photosynthetic vesicles for light-driven bioprocesses.
Journal Biotechnol. Lett.
Abstract We prepared ATP photosynthetic vesicles from inside-out membranes of Escherichia coli cells that express delta-rhodopsin (a novel light-driven H(+) transporter) and TF(0)F(1)-ATP synthase (a thermo-stable ATP synthase). These vesicles showed light-dependent ATP synthesis. Furthermore, coupling the ATP photosynthetic vesicles with an ATP-hydrolyzing hexokinase enabled light-dependent glucose consumption. The ATP photosynthetic vesicles indicate their potential to applied to light-driven ATP-regenerating bioprocess for various ATP-hydrolyzing bioproductions.
Volume 33(6)
Pages 1133-8
Published 2011-6
DOI 10.1007/s10529-011-0544-5
PMID 21287230
MeSH Adenosine Triphosphate / metabolism* Bacterial Proton-Translocating ATPases / genetics Bacterial Proton-Translocating ATPases / metabolism Biotechnology Escherichia coli / genetics Escherichia coli / metabolism Halobacteriaceae / genetics Halobacteriaceae / metabolism Hexokinase / metabolism Photosynthesis / genetics Photosynthesis / physiology* Recombinant Proteins / genetics Recombinant Proteins / metabolism Rhodopsins, Microbial / genetics Rhodopsins, Microbial / metabolism*
IF 2.154
Times Cited 4
General Microbes JCM 9743