RRC ID 47539
著者 Osburn MR, Amend JP.
タイトル Thermogladius shockii gen. nov., sp. nov., a hyperthermophilic crenarchaeote from Yellowstone National Park, USA.
ジャーナル Arch Microbiol
Abstract A hyperthermophilic heterotrophic archaeon (strain WB1) was isolated from a thermal pool in the Washburn hot spring group of Yellowstone National Park, USA. WB1 is a coccus, 0.6-1.2 μm in diameter, with a tetragonal S-layer, vacuoles, and occasional stalk-like protrusions. Growth is optimal at 84°C (range 64-93°C), pH 5-6 (range 3.5-8.5), and <1 g/l NaCl (range 0-4.6 g/l NaCl). Tests of metabolic properties show the isolate to be a strict anaerobe that ferments complex organic substrates. Phylogenetic analysis of the 16S rRNA gene sequence places WB1 in a clade of previously uncultured Desulfurococcaceae and shows it to have ≤ 96% 16S rRNA sequence identity to Desulfurococcus mobilis, Staphylothermus marinus, Staphylothermus hellenicus, and Sulfophobococcus zilligii. The 16S rRNA gene contains a large insertion similar to homing endonuclease introns reported in Thermoproteus and Pyrobaculum species. Growth is unaffected by the presence of S(0) or SO(4)(2-), thereby differentiating the isolate from its closest relatives. Based on phylogenetic and physiological differences, it is proposed that isolate WB1 represents the type strain of a novel genus and species within the Desulfurococcaceae, Thermogladius shockii gen. nov., sp. nov. (RIKEN = JCM-16579, ATCC = BAA-1607, Genbank 16S rRNA gene = EU183120).
巻・号 193(1)
ページ 45-52
公開日 2011-1-1
DOI 10.1007/s00203-010-0639-8
PMID 20978744
MeSH Base Composition DNA, Archaeal / chemistry Desulfurococcaceae / classification* Desulfurococcaceae / genetics Desulfurococcaceae / growth & development Desulfurococcaceae / isolation & purification Desulfurococcaceae / metabolism Hot Springs / microbiology* Hot Temperature Molecular Sequence Data Phenotype Phylogeny RNA, Ribosomal, 16S / genetics United States
IF 1.884
引用数 10
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 16579