論文 - 詳細
| RRC ID | 47539 |
|---|---|
| 著者 | Osburn MR, Amend JP. |
| タイトル | Thermogladius shockii gen. nov., sp. nov., a hyperthermophilic crenarchaeote from Yellowstone National Park, USA. |
| ジャーナル | Arch Microbiol |
| Abstract |
A hyperthermophilic heterotrophic archaeon (strain WB1) was isolated from a thermal pool in the Washburn hot spring group of Yellowstone National Park, USA. WB1 is a coccus, 0.6-1.2 μm in diameter, with a tetragonal S-layer, vacuoles, and occasional stalk-like protrusions. Growth is optimal at 84°C (range 64-93°C), pH 5-6 (range 3.5-8.5), and <1 g/l NaCl (range 0-4.6 g/l NaCl). Tests of metabolic properties show the isolate to be a strict anaerobe that ferments complex organic substrates. Phylogenetic analysis of the 16S rRNA gene sequence places WB1 in a clade of previously uncultured Desulfurococcaceae and shows it to have ≤ 96% 16S rRNA sequence identity to Desulfurococcus mobilis, Staphylothermus marinus, Staphylothermus hellenicus, and Sulfophobococcus zilligii. The 16S rRNA gene contains a large insertion similar to homing endonuclease introns reported in Thermoproteus and Pyrobaculum species. Growth is unaffected by the presence of S(0) or SO(4)(2-), thereby differentiating the isolate from its closest relatives. Based on phylogenetic and physiological differences, it is proposed that isolate WB1 represents the type strain of a novel genus and species within the Desulfurococcaceae, Thermogladius shockii gen. nov., sp. nov. (RIKEN = JCM-16579, ATCC = BAA-1607, Genbank 16S rRNA gene = EU183120). |
| 巻・号 | 193(1) |
| ページ | 45-52 |
| 公開日 | 2011-1-1 |
| DOI | 10.1007/s00203-010-0639-8 |
| PMID | 20978744 |
| MeSH | Base Composition DNA, Archaeal / chemistry Desulfurococcaceae / classification* Desulfurococcaceae / genetics Desulfurococcaceae / growth & development Desulfurococcaceae / isolation & purification Desulfurococcaceae / metabolism Hot Springs / microbiology* Hot Temperature Molecular Sequence Data Phenotype Phylogeny RNA, Ribosomal, 16S / genetics United States |
| IF | 1.884 |
| 引用数 | 10 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 16579 |