RRC ID 47555
Author Tohno M, Kobayashi H, Nomura M, Uegaki R, Cai Y.
Title Identification and characterization of lactic acid bacteria isolated from mixed pasture of timothy and orchardgrass, and its badly preserved silage.
Journal Anim Sci J
Abstract In order to understand the relationship between lactic acid bacteria (LAB) species and silage fermentation, a total of 65 LAB strains isolated from mixed pasture of timothy (Phleum pratense L.) and orchardgrass (Dactylis glomerata L.), and its badly preserved silages were subjected to phenotypic and genetic analysis. According to these analyses, the isolates were divided into 13 groups, including Enterococcus gallinarum, Lactobacillus acidipiscis, L. coryniformis subsp. coryniformis, L. coryniformis subsp. torquens, L. curvatus, L. paraplantarum, L. plantarum subsp. argentoratensis, L. plantarum subsp. plantarum, L. sakei subsp. carnosus, Lactococcus garvieae, Lactococcus lactis subsp. cremoris, Leuconostoc pseudomesenteroides, Pediococcus acidilactici, Pediococcus pentosaceus, Weissella hellenica, Weissella paramesenteroides and Carnobacterium divergens. This is the first report to document that C. divergens, L. acidipiscis, L. sakei subsp. carnosus, L. garvieae, phenotypically novel L. lactis subsp. cremoris, E. gallinarum and W. hellenica are present in vegetative forage crops. L. plantarum group strains were most frequently isolated from the badly preserved silages. Some isolates showed a wide range of growth preferences for carbohydrate utilization, optimal growth pH and temperature in vitro, indicating that they have a high growth potential. These results are useful in understanding the diversity of LAB associated with decayed silage of timothy and orchardgrass.
Volume 83(4)
Pages 318-30
Published 2012-4-1
DOI 10.1111/j.1740-0929.2011.00955.x
PMID 22515692
MeSH Dactylis / microbiology* Lactobacillales / isolation & purification* Molecular Sequence Data Phleum / microbiology* Polymerase Chain Reaction Silage / microbiology* Silage / poisoning*
IF 1.399
Times Cited 27
General Microbes JCM 10343 JCM 5805  JCM 16167  JCM 1180 JCM 1558 JCM 1149 JCM 16169 JCM 1096 JCM 1157 JCM 11031