論文 - 詳細
| RRC ID | 47570 |
|---|---|
| 著者 | Teramoto H, Inui M, Yukawa H. |
| タイトル | Corynebacterium glutamicum CsoR acts as a transcriptional repressor of two copper/zinc-inducible P(1B)-type ATPase operons. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
The mechanism of regulation of the expression of copA and copB, encoding putative copper-translocating P(1B)-type ATPases in Corynebacterium glutamicum, was investigated. The levels of copA and copB mRNAs were upregulated in response to excess copper as well as excess zinc. Disruption of csoR, encoding a transcriptional regulator, resulted in constitutive expression of copA and copB. The CsoR protein bound to the promoter regions of the copA-csoR and the cgR_0124-copB-cgR_0126 operon. In vitro DNA binding activity was strongly inhibited by copper, but much less inhibited by zinc. A csoR-deficient mutant showed slightly increased resistance to copper, but slightly decreased resistance to zinc. These findings indicate that CsoR acts as a transcriptional repressor not only of the cognate copA-csoR operon but also of the cgR_0124-copB-cgR_0126 operon, which is not physically linked to csoR on the chromosome, and that CsoR plays a major role in copper homeostasis. |
| 巻・号 | 76(10) |
| ページ | 1952-8 |
| 公開日 | 2012-1-1 |
| DOI | 10.1271/bbb.120437 |
| PII | DN/JST.JSTAGE/bbb/120437 |
| PMID | 23090582 |
| MeSH | Adenosine Triphosphatases / genetics* Adenosine Triphosphatases / metabolism Bacterial Proteins / metabolism* Base Sequence Biological Transport Copper / metabolism Copper / pharmacology* Corynebacterium glutamicum / enzymology Corynebacterium glutamicum / genetics Corynebacterium glutamicum / metabolism* DNA, Bacterial / metabolism Gene Expression Regulation, Bacterial / drug effects Gene Expression Regulation, Bacterial / genetics Genome, Bacterial / drug effects Genome, Bacterial / genetics Operon / drug effects* Operon / genetics Promoter Regions, Genetic / drug effects Promoter Regions, Genetic / genetics Repressor Proteins / metabolism* Zinc / pharmacology* |
| IF | 1.516 |
| 引用数 | 7 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 18229 |