論文 - 詳細
| RRC ID | 47593 |
|---|---|
| 著者 | Masuda Y, Zendo T, Sawa N, Perez RH, Nakayama J, Sonomoto K. |
| タイトル | Characterization and identification of weissellicin Y and weissellicin M, novel bacteriocins produced by Weissella hellenica QU 13. |
| ジャーナル | J Appl Microbiol |
| Abstract |
AIMS:To identify and characterize novel bacteriocins from Weissella hellenica QU 13. METHODS AND RESULTS:Weissella hellenica QU 13, isolated from a barrel used to make Japanese pickles, produced two novel bacteriocins termed weissellicin Y and weissellicin M. The primary structures of weissellicins Y and M were determined, and their molecular masses were determined to be 4925·12 and 4968·40 Da, respectively. Analysis of the DNA sequence encoding the bacteriocins revealed that they were synthesized and secreted without N-terminal extensions such as leader sequences or sec signal peptides. Weissellicin M showed significantly high and characteristic homology with enterocins L50A and L50B, produced by Enterococcus faecium L50, while weissellicin Y showed no homology with any other known bacteriocins. Both bacteriocins showed broad antimicrobial spectra, with especially high antimicrobial activity against species, which contaminate pickles, such as Bacillus coagulans, and weissellicin M showed relatively higher activity than weissellicin Y. Furthermore, the stability of weissellicin M against pH and heat was distinctively higher than that of weissellicin Y. CONCLUSIONS:Weissella hellenica QU 13 produced two novel leaderless bacteriocins, weissellicin Y and weissellicin M, and weissellicin M exhibited remarkable potency that could be employed by pickle-producing industry. SIGNIFICANCE AND IMPACT OF THE STUDY:This study is the first report, which represents a complete identification and characterization of novel leaderless bacteriocins from Weissella genus. |
| 巻・号 | 112(1) |
| ページ | 99-108 |
| 公開日 | 2012-1-1 |
| DOI | 10.1111/j.1365-2672.2011.05180.x |
| PMID | 22008177 |
| MeSH | Amino Acid Sequence Anti-Infective Agents / pharmacology Bacteria / drug effects Bacteriocins / chemistry* Bacteriocins / genetics* Bacteriocins / isolation & purification Base Sequence Gene Order Hot Temperature Hydrogen-Ion Concentration Mass Spectrometry Molecular Sequence Data Sequence Alignment Weissella / chemistry* Weissella / genetics Weissella / isolation & purification Weissella / physiology* |
| IF | 3.066 |
| 引用数 | 34 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 2257 JCM 7638 JCM 1157 JCM 1149 JCM 12495 JCM 10103 JCM 9890 JCM 1093 JCM 5885 JCM 5887 JCM 8797 JCM 5804 JCM 5803 JCM 5707 JCM 5802 JCM 5705 JCM 5673 JCM 2257 JCM 2504 JCM 1465 JCM 2152 JCM 6124 |