Reference - Detail
|Author||Matsu-Ura T, Dovzhenok A, Aihara E, Rood J, Le H, Ren Y, Rosselot AE, Zhang T, Lee C, Obrietan K, Montrose MH, Lim S, Moore SR, Hong CI.|
|Title||Intercellular Coupling of the Cell Cycle and Circadian Clock in Adult Stem Cell Culture.|
Circadian clock-gated cell division cycles are observed from cyanobacteria to mammals via intracellular molecular connections between these two oscillators. Here we demonstrate WNT-mediated intercellular coupling between the cell cycle and circadian clock in 3D murine intestinal organoids (enteroids). The circadian clock gates a population of cells with heterogeneous cell-cycle times that emerge as 12-hr synchronized cell division cycles. Remarkably, we observe reduced-amplitude oscillations of circadian rhythms in intestinal stem cells and progenitor cells, indicating an intercellular signal arising from differentiated cells governing circadian clock-dependent synchronized cell division cycles. Stochastic simulations and experimental validations reveal Paneth cell-secreted WNT as the key intercellular coupling component linking the circadian clock and cell cycle in enteroids.
|MeSH||Adult Stem Cells / physiology Animals Cell Cycle / physiology* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Circadian Clocks / physiology* Circadian Rhythm Intestinal Mucosa / physiology* Jejunum / metabolism Mice Nuclear Proteins / genetics Nuclear Proteins / metabolism Organoids Period Circadian Proteins / genetics Period Circadian Proteins / metabolism Protein-Tyrosine Kinases / genetics Protein-Tyrosine Kinases / metabolism Tissue Culture Techniques Wnt Signaling Pathway / physiology*|
|WOS Category||BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY|
|DNA material||CSII-EF-MCS (RDB04378)|