論文 - 詳細
RRC ID | 47633 |
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著者 | Matsu-Ura T, Dovzhenok A, Aihara E, Rood J, Le H, Ren Y, Rosselot AE, Zhang T, Lee C, Obrietan K, Montrose MH, Lim S, Moore SR, Hong CI. |
タイトル | Intercellular Coupling of the Cell Cycle and Circadian Clock in Adult Stem Cell Culture. |
ジャーナル | Mol Cell |
Abstract |
Circadian clock-gated cell division cycles are observed from cyanobacteria to mammals via intracellular molecular connections between these two oscillators. Here we demonstrate WNT-mediated intercellular coupling between the cell cycle and circadian clock in 3D murine intestinal organoids (enteroids). The circadian clock gates a population of cells with heterogeneous cell-cycle times that emerge as 12-hr synchronized cell division cycles. Remarkably, we observe reduced-amplitude oscillations of circadian rhythms in intestinal stem cells and progenitor cells, indicating an intercellular signal arising from differentiated cells governing circadian clock-dependent synchronized cell division cycles. Stochastic simulations and experimental validations reveal Paneth cell-secreted WNT as the key intercellular coupling component linking the circadian clock and cell cycle in enteroids. |
巻・号 | 64(5) |
ページ | 900-912 |
公開日 | 2016-12-1 |
DOI | 10.1016/j.molcel.2016.10.015 |
PII | S1097-2765(16)30638-4 |
PMID | 27867006 |
PMC | PMC5423461 |
MeSH | Adult Stem Cells / physiology Animals Cell Cycle / physiology* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Circadian Clocks / physiology* Circadian Rhythm Intestinal Mucosa / physiology* Jejunum / metabolism Mice Nuclear Proteins / genetics Nuclear Proteins / metabolism Organoids Period Circadian Proteins / genetics Period Circadian Proteins / metabolism Protein-Tyrosine Kinases / genetics Protein-Tyrosine Kinases / metabolism Tissue Culture Techniques Wnt Signaling Pathway / physiology* |
IF | 15.584 |
引用数 | 34 |
WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
リソース情報 | |
遺伝子材料 | CSII-EF-MCS (RDB04378) |