論文 - 詳細
| RRC ID | 47672 |
|---|---|
| 著者 | Nakazawa M, Matsubara H, Matsushita Y, Watanabe M, Vo N, Yoshida H, Yamaguchi M, Kataoka T. |
| タイトル | The Human Bcl-2 Family Member Bcl-rambo Localizes to Mitochondria and Induces Apoptosis and Morphological Aberrations in Drosophila. |
| ジャーナル | PLoS One |
| Abstract |
Bcl-2 family proteins play a central role in regulating apoptosis. We previously reported that human Bcl-rambo, also termed BCL2L13, localized to mitochondria and induced apoptosis when overexpressed in human embryonic kidney 293T cells. However, the physiological function of Bcl-rambo currently remains unclear. In the present study, human Bcl-rambo was ectopically expressed in Drosophila melanogaster. Bcl-rambo mainly localized to the mitochondria of Drosophila Schneider 2 (S2) cells. The overexpression of Bcl-rambo, but not Bcl-rambo lacking a C-terminal transmembrane domain, induced apoptosis in S2 cells. Moreover, the ectopic expression of Bcl-rambo by a GAL4-UAS system induced aberrant morphological changes characterized by atrophied wing, split thorax, and rough eye phenotypes. Bcl-rambo induced the activation of effector caspases in eye imaginal discs. The rough eye phenotype induced by Bcl-rambo was partly rescued by the co-expression of p35, Diap1, and Diap2. By using this Drosophila model, we showed that human Bcl-rambo interacted genetically with Drosophila homologues of adenine nucleotide translocators and the autophagy-related 8 protein. The results of the present study demonstrated that human Bcl-rambo localized to mitochondria and at least regulated an apoptosis signaling pathway in Drosophila. |
| 巻・号 | 11(6) |
| ページ | e0157823 |
| 公開日 | 2016-6-27 |
| DOI | 10.1371/journal.pone.0157823 |
| PII | PONE-D-15-24486 |
| PMID | 27348811 |
| PMC | PMC4922555 |
| MeSH | Animals Apoptosis* Autophagy-Related Protein 8 Family / genetics Cell Line Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / genetics* Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism Epistasis, Genetic Humans Inhibitor of Apoptosis Proteins / genetics Inhibitor of Apoptosis Proteins / metabolism Mitochondria / metabolism* Mitochondrial ADP, ATP Translocases / genetics Phenotype* Protein Transport Proto-Oncogene Proteins c-bcl-2 / genetics* Proto-Oncogene Proteins c-bcl-2 / metabolism |
| IF | 2.74 |
| 引用数 | 15 |
| WOS 分野 | CELL BIOLOGY |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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