RRC ID 47692
Author Suzuki K, Kimura T, Shinoda H, Bai G, Daniels MJ, Arai Y, Nakano M, Nagai T.
Title Five colour variants of bright luminescent protein for real-time multicolour bioimaging.
Journal Nat Commun
Abstract Luminescence imaging has gained attention as a promising bio-imaging modality in situations where fluorescence imaging cannot be applied. However, wider application to multicolour and dynamic imaging is limited by the lack of bright luminescent proteins with emissions across the visible spectrum. Here we report five new spectral variants of the bright luminescent protein, enhanced Nano-lantern (eNL), made by concatenation of the brightest luciferase, NanoLuc, with various colour hues of fluorescent proteins. eNLs allow five-colour live-cell imaging, as well as detection of single protein complexes and even single molecules. We also develop an eNL-based Ca2+ indicator with a 500% signal change, which can image spontaneous Ca2+ dynamics in cardiomyocyte and neural cell models. These eNL probes facilitate not only multicolour imaging in living cells but also sensitive imaging of a wide repertoire of proteins, even at very low expression levels.
Volume 7
Pages 13718
Published 2016-12-14
DOI 10.1038/ncomms13718
PII ncomms13718
PMID 27966527
PMC PMC5171807
MeSH Calcium / analysis Calcium Signaling Color* HeLa Cells Humans Luminescent Proteins / analysis Luminescent Proteins / chemistry* Myocytes, Cardiac / metabolism Neurons / metabolism Optical Imaging / methods
IF 12.121
Times Cited 59
Human and Animal Cells 293T(RCB2202) 201B7(HPS0063) HeLa(RCB3680)
DNA material ReNL/pcDNA3 (RDB19870) OeNL/pcDNA3 (RDB19871) YeNL/pcDNA3 (RDB19872) GeNL/pcDNA3 (RDB19873) CeNL/pcDNA3 (RDB19874)