論文 - 詳細
| RRC ID | 47692 |
|---|---|
| 著者 | Suzuki K, Kimura T, Shinoda H, Bai G, Daniels MJ, Arai Y, Nakano M, Nagai T. |
| タイトル | Five colour variants of bright luminescent protein for real-time multicolour bioimaging. |
| ジャーナル | Nat Commun |
| Abstract |
Luminescence imaging has gained attention as a promising bio-imaging modality in situations where fluorescence imaging cannot be applied. However, wider application to multicolour and dynamic imaging is limited by the lack of bright luminescent proteins with emissions across the visible spectrum. Here we report five new spectral variants of the bright luminescent protein, enhanced Nano-lantern (eNL), made by concatenation of the brightest luciferase, NanoLuc, with various colour hues of fluorescent proteins. eNLs allow five-colour live-cell imaging, as well as detection of single protein complexes and even single molecules. We also develop an eNL-based Ca2+ indicator with a 500% signal change, which can image spontaneous Ca2+ dynamics in cardiomyocyte and neural cell models. These eNL probes facilitate not only multicolour imaging in living cells but also sensitive imaging of a wide repertoire of proteins, even at very low expression levels. |
| 巻・号 | 7 |
| ページ | 13718 |
| 公開日 | 2016-12-14 |
| DOI | 10.1038/ncomms13718 |
| PII | ncomms13718 |
| PMID | 27966527 |
| PMC | PMC5171807 |
| MeSH | Calcium / analysis Calcium Signaling Color* HeLa Cells Humans Luminescent Proteins / analysis Luminescent Proteins / chemistry* Myocytes, Cardiac / metabolism Neurons / metabolism Optical Imaging / methods |
| IF | 12.121 |
| 引用数 | 59 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 85 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 6.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) 201B7(HPS0063) HeLa |
| 遺伝子材料 | ReNL/pcDNA3 (RDB19870) OeNL/pcDNA3 (RDB19871) YeNL/pcDNA3 (RDB19872) GeNL/pcDNA3 (RDB19873) CeNL/pcDNA3 (RDB19874) |