RRC ID 477
Author Daimon T, Hamada K, Mita K, Okano K, Suzuki MG, Kobayashi M, Shimada T.
Title A Bombyx mori gene, BmChi-h, encodes a protein homologous to bacterial and baculovirus chitinases.
Journal Insect Biochem. Mol. Biol.
Abstract We have cloned and characterized a novel chitinase gene (BmChi-h) from the silkworm, Bombyx mori. BmChi-h cDNA has an open reading frame of 1,665 nucleotides, encoding a protein of 555 amino acid residues. The predicted protein shared extensive similarities with bacterial and baculovirus chitinases in both amino acid sequences (73% identity with Serratia marcescens chiA and 63% with Autographa californica nucleopolyhedrovirus chiA) and domain architectures. BmChi-h was a single-copy gene and located on chromosome 7. The expression of BmChi-h mRNA was observed in a stage- and tissue-specific manner that was almost identical to that of another chitinase gene previously cloned from B. mori. We further determined the overall genomic organization of BmChi-h. There was no intron in the ORF of BmChi-h. However, BmChi-h was transcribed from three promoters, which generated three isoforms in the 5'-UTR of the transcript. Phylogenetic analysis suggested that ancestral species of B. mori acquired the chitinase gene from a bacterium or an ancestral baculovirus via horizontal gene transfer.
Volume 33(8)
Pages 749-59
Published 2003-8
PII S0965174803000845
PMID 12878222
MeSH Amino Acid Sequence Animals Bacterial Proteins / genetics* Baculoviridae / chemistry Baculoviridae / genetics* Base Sequence Biological Evolution Blotting, Northern Blotting, Southern Bombyx / genetics* Chitinases / genetics* Cloning, Molecular DNA, Complementary Gene Transfer, Horizontal Insect Proteins / genetics* Molecular Sequence Data Phylogeny Polymerase Chain Reaction Sequence Homology, Amino Acid
IF 3.562
Times Cited 52
WOS Category ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Silkworms p50