論文 - 詳細
| RRC ID | 4770 |
|---|---|
| 著者 | Kobayashi N, Noguchi H, Westerman KA, Watanabe T, Matsumura T, Totsugawa T, Fujiwara T, Leboulch P, Tanaka N. |
| タイトル | Cre/loxP-based reversible immortalization of human hepatocytes. |
| ジャーナル | Cell Transplant |
| Abstract |
An ideal alternative to the primary human hepatocytes for hepatocyte transplantation would be to use a clonal cell line that grows economically in culture and exhibits the characteristics of differentiated, nontransformed hepatocytes following transplantation. The purpose of the present studies was to establish a reversibly immortalized human hepatocyte cell line. Human hepatocytes were immortalized with a retroviral vector SSR#69 expressing simian virus 40 large T antigen (SV40Tag) gene flanked by a pair of loxP recombination targets. One of the resulting clones, NKNT-3, showed morphological characteristics of liver parenchymal cells and expressed the genes of differentiated liver functions. NKNT-3 cells offered unlimited availability. After an adenoviral delivery of Cre recombinase and subsequent differential selection, efficient removal of SV40Tag from NKNT-3 cells was performed. Here we represent that elimination of the retrovirally transferred SV40Tag gene can be excised by adenovirus-mediated site-specific recombination. |
| 巻・号 | 10(4-5) |
| ページ | 383-6 |
| 公開日 | 2001-1-1 |
| PMID | 11549058 |
| MeSH | Cell Line* Cell Transplantation Culture Media, Serum-Free Gene Transfer Techniques* Genetic Vectors Hepatocytes / physiology* Hepatocytes / transplantation Humans Integrases / metabolism* Liver / physiology Recombination, Genetic Retroviridae / genetics Retroviridae / metabolism Transduction, Genetic Viral Proteins / metabolism* |
| IF | 3.341 |
| 引用数 | 17 |
| WOS 分野 | TRANSPLANTATION MEDICINE, RESEARCH & EXPERIMENTAL CELL & TISSUE ENGINEERING |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | AxCALacZ (RDB1745) |