論文 - 詳細
| RRC ID | 47700 |
|---|---|
| 著者 | Goparaju SK, Kohda K, Ibata K, Soma A, Nakatake Y, Akiyama T, Wakabayashi S, Matsushita M, Sakota M, Kimura H, Yuzaki M, Ko SB, Ko MS. |
| タイトル | Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors. |
| ジャーナル | Sci Rep |
| Abstract |
Efficient differentiation of human pluripotent stem cells (hPSCs) into neurons is paramount for disease modeling, drug screening, and cell transplantation therapy in regenerative medicine. In this manuscript, we report the capability of five transcription factors (TFs) toward this aim: NEUROG1, NEUROG2, NEUROG3, NEUROD1, and NEUROD2. In contrast to previous methods that have shortcomings in their speed and efficiency, a cocktail of these TFs as synthetic mRNAs can differentiate hPSCs into neurons in 7 days, judged by calcium imaging and electrophysiology. They exhibit motor neuron phenotypes based on immunostaining. These results indicate the establishment of a novel method for rapid, efficient, and footprint-free differentiation of functional neurons from hPSCs. |
| 巻・号 | 7 |
| ページ | 42367 |
| 公開日 | 2017-2-13 |
| DOI | 10.1038/srep42367 |
| PII | srep42367 |
| PMID | 28205555 |
| PMC | PMC5304326 |
| MeSH | Biomarkers / metabolism Cell Differentiation / genetics* Cell Shape Humans Ion Channels / metabolism Kinetics Motor Neurons / cytology* Motor Neurons / metabolism Neurogenesis / genetics Pluripotent Stem Cells / cytology* Pluripotent Stem Cells / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism Transcription Factors / genetics* Transcription Factors / metabolism |
| IF | 3.998 |
| 引用数 | 26 |
| WOS 分野 | NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 21 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) |