論文 - 詳細
| RRC ID | 47719 |
|---|---|
| 著者 | Kanatsu-Shinohara M, Tanaka T, Ogonuki N, Ogura A, Morimoto H, Cheng PF, Eisenman RN, Trumpp A, Shinohara T. |
| タイトル | Myc/Mycn-mediated glycolysis enhances mouse spermatogonial stem cell self-renewal. |
| ジャーナル | Genes Dev |
| Abstract |
Myc plays critical roles in the self-renewal division of various stem cell types. In spermatogonial stem cells (SSCs), Myc controls SSC fate decisions because Myc overexpression induces enhanced self-renewal division, while depletion of Max, a Myc-binding partner, leads to meiotic induction. However, the mechanism by which Myc acts on SSC fate is unclear. Here we demonstrate a critical link between Myc/Mycn gene activity and glycolysis in SSC self-renewal. In SSCs, Myc/Mycn are regulated by Foxo1, whose deficiency impairs SSC self-renewal. Myc/Mycn-deficient SSCs not only undergo limited self-renewal division but also display diminished glycolytic activity. While inhibition of glycolysis decreased SSC activity, chemical stimulation of glycolysis or transfection of active Akt1 or Pdpk1 (phosphoinositide-dependent protein kinase 1 ) augmented self-renewal division, and long-term SSC cultures were derived from a nonpermissive strain that showed limited self-renewal division. These results suggested that Myc-mediated glycolysis is an important factor that increases the frequency of SSC self-renewal division. |
| 巻・号 | 30(23) |
| ページ | 2637-2648 |
| 公開日 | 2016-12-1 |
| DOI | 10.1101/gad.287045.116 |
| PII | 30/23/2637 |
| PMID | 28007786 |
| PMC | PMC5204355 |
| MeSH | 3-Phosphoinositide-Dependent Protein Kinases / metabolism Animals Cell Division / genetics Cell Proliferation / genetics Cell Self Renewal / genetics* Gene Expression Regulation, Developmental / genetics* Gene Knockout Techniques Glycolysis / genetics* Male Mice Mice, Inbred C57BL N-Myc Proto-Oncogene Protein / genetics N-Myc Proto-Oncogene Protein / metabolism* Proto-Oncogene Proteins c-myc / genetics Proto-Oncogene Proteins c-myc / metabolism* RNA Splicing Factors / metabolism Spermatogonia / cytology* Stem Cells / enzymology Stem Cells / metabolism* |
| IF | 9.527 |
| 引用数 | 20 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY GENETICS & HEREDITY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 23 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | AxCANCre (RDB01748) AxCANLacZ (RDB01749). |