RRC ID 47723
Author Sugimoto H, Kusumi K, Tozawa Y, Yazaki J, Kishimoto N, Kikuchi S, Iba K.
Title The virescent-2 mutation inhibits translation of plastid transcripts for the plastid genetic system at an early stage of chloroplast differentiation.
Journal Plant Cell Physiol
Abstract The rice virescent-2 mutant (v(2)) is temperature conditional and develops chlorotic, chloroplast-deficient leaves at the restrictive temperature. In the v(2) mutant, plastid-encoded proteins involved in photosynthesis and plastid transcriptional regulation were not detectable at any time during chloroplast differentiation. However, the plastid transcripts for these two classes of proteins behaved differently in the mutant, with those for the plastid transcription/translation apparatus accumulating to wild-type levels and those for photosynthetic apparatus being suppressed. Polysome analysis showed that translation of the plastid transcripts encoding the plastid transcription/translation apparatus was blocked at an early stage of chloroplast differentiation. Accumulation of transcripts of nuclear-encoded photosynthetic genes, such as cab and rbcS, was strongly suppressed in the mutant at later stages of chloroplast differentiation, whereas transcripts of genes for the plastid transcription apparatus, such as OsRpoTp and OsSIG2A, accumulated to abnormally high levels at these stages. These results suggest that activation of the plastid translation machinery at an early stage of chloroplast differentiation is important for triggering the transmission of information about plastid developmental state to the nucleus, which in turn is required for the induction of nuclear-encoded chloroplast proteins at later stages of chloroplast differentiation.
Volume 45(8)
Pages 985-96
Published 2004-8-1
DOI 10.1093/pcp/pch111
PII 45/8/985
PMID 15356324
MeSH Cell Differentiation / genetics Cell Nucleus / genetics DNA-Directed RNA Polymerases / genetics Down-Regulation / genetics Gene Expression Regulation, Plant / genetics Genes, Regulator / genetics Light-Harvesting Protein Complexes / genetics Light-Harvesting Protein Complexes / metabolism Molecular Sequence Data Mutation / genetics* Oryza / cytology Oryza / genetics* Oryza / growth & development Photosynthesis / genetics Plant Proteins / biosynthesis* Plant Proteins / genetics Plastids / genetics* Plastids / metabolism Protein Biosynthesis / genetics* Ribulose-Bisphosphate Carboxylase / genetics Ribulose-Bisphosphate Carboxylase / metabolism Silencer Elements, Transcriptional / genetics
IF 4.062
Times Cited 77
Rice 標識遺伝子系統