Reference - Detail
| RRC ID | 4774 |
|---|---|
| Author | Noguchi M, Imaizumi K, Kawabe T, Wakayama H, Horio Y, Sekido Y, Hara T, Hashimoto N, Takahashi M, Shimokata K, Hasegawa Y. |
| Title | Induction of antitumor immunity by transduction of CD40 ligand gene and interferon-gamma gene into lung cancer. |
| Journal | Cancer Gene Ther |
| Abstract |
CD40-CD40 ligand (CD40L) interaction is an important costimulatory signaling pathway in the crosstalk between T cells and antigen-presenting cells. This receptor-ligand system is known to be essential in eliciting strong cellular immunity. Here we demonstrate that murine lung cancer cells (3LLSA) transduced with the CD40L gene (3LLSA-CD40L) were rejected in syngeneic C57BL/6 mice, but grew in CD40-deficient mice to the same extent as control tumor cells. Immunohistochemical study showed that inflammatory cells, including CD4+, CD8+ T cells and NK cells, infiltrated into the inoculated 3LLSA-CD40L tumor tissue. Inoculation of 3LLSA-CD40L cells into mice resulted in the induction of 3LLSA-specific cytotoxic T-cell immunity, and the growth of parental 3LLSA tumors was inhibited when 3LLSA cells were inoculated into C57BL/6 mice mixed with 3LLSA-CD40L cells or when they were rechallenged 4 weeks after 3LLSA-CD40L cells were rejected. Furthermore, co-inoculation of interferon (IFN)-gamma-transduced cells (3LLSA-IFNgamma) with 3LLSA-CD40L cells enhanced the antitumor immunity efficiently in vivo. These results indicate that the in vivo priming with CD40L- and IFN-gamma gene-transduced lung cancer cells is a promising strategy for inducing antitumor immunity in the treatment of lung cancer. |
| Volume | 8(6) |
| Pages | 421-9 |
| Published | 2001-6-1 |
| DOI | 10.1038/sj.cgt.7700320 |
| PMID | 11498762 |
| MeSH | Animals CD40 Ligand / genetics* Cancer Vaccines* DNA, Complementary / metabolism Genetic Therapy / methods* Immunohistochemistry Interferon-gamma / genetics* Interferon-gamma / metabolism Lung Neoplasms / genetics* Lung Neoplasms / therapy* Mice Mice, Inbred C57BL Recombinant Proteins / metabolism Spleen / metabolism T-Lymphocytes, Cytotoxic / metabolism Time Factors Transduction, Genetic* Transfection Tumor Cells, Cultured |
| IF | 4.534 |
| Times Cited | 21 |
| WOS Category | MEDICINE, RESEARCH & EXPERIMENTAL BIOTECHNOLOGY & APPLIED MICROBIOLOGY ONCOLOGY GENETICS & HEREDITY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pBluescript KS+mIFN gamma (RDB01482) |
| Human and Animal Cells | B16 melanoma 4A5(RCB0557) |