RRC ID 47768
著者 Fernandez-Moreno JP, Tzfadia O, Forment J, Presa S, Rogachev I, Meir S, Orzaez D, Aharoni A, Granell A.
タイトル Characterization of a New Pink-Fruited Tomato Mutant Results in the Identification of a Null Allele of the SlMYB12 Transcription Factor.
ジャーナル Plant Physiol
Abstract The identification and characterization of new tomato (Solanum lycopersicum) mutants affected in fruit pigmentation and nutritional content can provide valuable insights into the underlying biology, as well as a source of new alleles for breeding programs. To date, all characterized pink-pigmented tomato fruit mutants appear to result from low SlMYB12 transcript levels in the fruit skin. Two new mutant lines displaying a pink fruit phenotype (pf1 and pf2) were characterized in this study. In the pf mutants, SlMYB12 transcripts accumulated to wild-type levels but exhibited the same truncation, which resulted in the absence of the essential MYB activation domain coding region. Allelism and complementation tests revealed that both pf mutants were allelic to the y locus and showed the same recessive null allele in homozygosis: Δy A set of molecular and metabolic effects, reminiscent of those observed in the Arabidopsis (Arabidopsis thaliana) myb11 myb12 myb111 triple mutant, were found in the tomato Δy mutants. To our knowledge, these have not been described previously, and our data support the idea of their being null mutants, in contrast to previously described transcriptional hypomorphic pink fruit lines. We detected a reduction in the expression of several flavonol glycosides and some associated glycosyl transferases. Transcriptome analysis further revealed that the effects of the pf mutations extended beyond the flavonoid pathway into the interface between primary and secondary metabolism. Finally, screening for Myb-binding sites in the candidate gene promoter sequences revealed that 141 of the 152 co-down-regulated genes may be direct targets of SlMYB12 regulation.
巻・号 171(3)
ページ 1821-36
公開日 2016-7-1
DOI 10.1104/pp.16.00282
PII pp.16.00282
PMID 27208285
PMC PMC4936558
MeSH Alleles Chromatography, Liquid Flavonoids / biosynthesis Flavonoids / genetics Flavonols / metabolism Fruit / genetics Fruit / physiology* Gene Expression Regulation, Plant Glycosylation Mass Spectrometry / methods Metabolomics / methods Mutation Pigmentation / genetics Plant Proteins / genetics* Plant Proteins / metabolism Plants, Genetically Modified / genetics Sequence Analysis, RNA Solanum lycopersicum / genetics* Solanum lycopersicum / physiology Transcription Factors / genetics* Transcription Factors / metabolism
IF 6.902
引用数 13
WOS 分野 PLANT SCIENCES
リソース情報
トマト TOMJPF00001 TOMJPG4497-1 TOMJPG5663-1