RRC ID 47769
著者 Shimatani Z, Kashojiya S, Takayama M, Terada R, Arazoe T, Ishii H, Teramura H, Yamamoto T, Komatsu H, Miura K, Ezura H, Nishida K, Ariizumi T, Kondo A.
タイトル Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion.
ジャーナル Nat Biotechnol
Abstract We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.
巻・号 35(5)
ページ 441-443
公開日 2017-5-1
DOI 10.1038/nbt.3833
PII nbt.3833
PMID 28346401
MeSH Base Pairing / genetics CRISPR-Associated Proteins / genetics* Clustered Regularly Interspaced Short Palindromic Repeats / genetics Cytidine Deaminase / genetics DNA, Plant / genetics* Gene Editing / methods* Genes, Plant / genetics Mutagenesis, Site-Directed / methods* Oryza / genetics* Plants, Genetically Modified / genetics Point Mutation / genetics Recombinant Fusion Proteins / genetics Solanum lycopersicum / genetics*
IF 36.553
引用数 189
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
トマト TOMJPF00001