| RRC ID |
4777
|
| Author |
Takatsu H, Tasaki H, Kim HN, Ueda S, Tsutsui M, Yamashita K, Toyokawa T, Morimoto Y, Nakashima Y, Adachi T.
|
| Title |
Overexpression of EC-SOD suppresses endothelial-cell-mediated LDL oxidation.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Reactive oxygen species have been proposed to play important roles in atherosclerosis. To investigate the protective role of extracellular superoxide dismutase (EC-SOD), its inhibition of endothelial-cell-mediated LDL oxidation was examined. We constructed the recombinant adenovirus AxCAEC-SOD expressing human EC-SOD by CAG promoter. Infection of endothelial cells with AxCAEC-SOD resulted in EC-SOD protein secretion in a dose-dependent manner and a decrease of endothelial-cell-derived superoxide production. Moreover, it was proven to coexist with heparan sulfate by immunohistochemical staining. Endothelial-cell-mediated LDL oxidation enhanced by ferric-sodium EDTA was inhibited by 47% in TBARS formation by AxCAEC-SOD infection. In agarose gel electrophoresis, AxCAEC-SOD decreased the negative charge of oxidized LDL by 50% and suppressed fragmentation of apolipoprotein B. These results suggested that human EC-SOD localized in the extracellular space and reduced endothelial-cell-mediated LDL oxidation. In subendothelial space, EC-SOD bound on heparan sulfate might suppress LDL oxidation through reduction of superoxide anion.
|
| Volume |
285(1)
|
| Pages |
84-91
|
| Published |
2001-7-6
|
| DOI |
10.1006/bbrc.2001.5114
|
| PII |
S0006-291X(01)95114-9
|
| PMID |
11437376
|
| MeSH |
Animals
Base Sequence
DNA Primers
Electrophoresis, Polyacrylamide Gel
Endothelium, Vascular / enzymology*
Endothelium, Vascular / physiology*
Heparitin Sulfate / metabolism
Immunohistochemistry
Kinetics
Lipoproteins, LDL / metabolism*
Oxidation-Reduction
Recombinant Proteins / metabolism
Superoxide Dismutase / metabolism*
Swine
|
| IF |
2.985
|
| Times Cited |
44
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
pAxCAwt (RDB1678) |
