論文 - 詳細
| RRC ID | 47898 |
|---|---|
| 著者 | Sumiya N, Fujiwara T, Era A, Miyagishima SY. |
| タイトル | Chloroplast division checkpoint in eukaryotic algae. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Chloroplasts evolved from a cyanobacterial endosymbiont. It is believed that the synchronization of endosymbiotic and host cell division, as is commonly seen in existing algae, was a critical step in establishing the permanent organelle. Algal cells typically contain one or only a small number of chloroplasts that divide once per host cell cycle. This division is based partly on the S-phase-specific expression of nucleus-encoded proteins that constitute the chloroplast-division machinery. In this study, using the red alga Cyanidioschyzon merolae, we show that cell-cycle progression is arrested at the prophase when chloroplast division is blocked before the formation of the chloroplast-division machinery by the overexpression of Filamenting temperature-sensitive (Fts) Z2-1 (Fts72-1), but the cell cycle progresses when chloroplast division is blocked during division-site constriction by the overexpression of either FtsZ2-1 or a dominant-negative form of dynamin-related protein 5B (DRP5B). In the cells arrested in the prophase, the increase in the cyclin B level and the migration of cyclin-dependent kinase B (CDKB) were blocked. These results suggest that chloroplast division restricts host cell-cycle progression so that the cell cycle progresses to the metaphase only when chloroplast division has commenced. Thus, chloroplast division and host cell-cycle progression are synchronized by an interactive restriction that takes place between the nucleus and the chloroplast. In addition, we observed a similar pattern of cell-cycle arrest upon the blockage of chloroplast division in the glaucophyte alga Cyanophora paradoxa, raising the possibility that the chloroplast division checkpoint contributed to the establishment of the permanent organelle. |
| 巻・号 | 113(47) |
| ページ | E7629-E7638 |
| 公開日 | 2016-11-22 |
| DOI | 10.1073/pnas.1612872113 |
| PII | 1612872113 |
| PMID | 27837024 |
| PMC | PMC5127316 |
| MeSH | Cell Cycle Cell Nucleus / genetics Cell Nucleus / metabolism Chloroplasts / physiology* Plant Proteins / genetics Plant Proteins / metabolism* Rhodophyta / physiology* Up-Regulation |
| IF | 9.412 |
| 引用数 | 13 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-547 NIES-3377 |