RRC ID 4790
Author Akihiro T, Koike S, Tani R, Tominaga T, Watanabe S, Iijima Y, Aoki K, Shibata D, Ashihara H, Matsukura C, Akama K, Fujimura T, Ezura H.
Title Biochemical mechanism on GABA accumulation during fruit development in tomato.
Journal Plant Cell Physiol.
Abstract A large amount of gamma-aminobutyric acid (GABA) was found to accumulate in tomato (Solanum lycopersicum) fruits before the breaker stage. Shortly thereafter, GABA was rapidly catabolized after the breaker stage. We screened the GABA-rich tomato cultivar 'DG03-9' which did not show rapid GABA catabolism after the breaker stage. Although GABA hyperaccumulation and rapid catabolism in fruits is well known, the mechanisms are not clearly understood. In order to clarify these mechanisms, we performed comparative studies of 'Micro-Tom' and 'DG03-9' fruits for the analysis of gene expression levels, protein levels and enzymatic activity levels of GABA biosynthesis- and catabolism-related enzymes. During GABA accumulation, we found positive correlations among GABA contents and expression levels of SlGAD2 and SlGAD3. Both of these genes encode glutamate decarboxylase (GAD) which is a key enzyme of GABA biosynthesis. During GABA catabolism, we found a strong correlation between GABA contents and enzyme activity of alpha-ketoglutarate-dependent GABA transaminase (GABA-TK). The contents of glutamate and aspartate, which are synthesized from GABA and glutamate, respectively, increased with elevation of GABA-TK enzymatic activity. GABA-TK is the major GABA transaminase form in animals and appears to be a minor form in plants. In 'DG03-9' fruits, GAD enzymatic activity was prolonged until the ripening stage, and GABA-TK activity was significantly low. Taken together, our results suggest that GAD and GABA-TK play crucial roles in GABA accumulation and catabolism, respectively, in tomato fruits.
Volume 49(9)
Pages 1378-89
Published 2008-9
DOI 10.1093/pcp/pcn113
PII pcn113
PMID 18713763
MeSH 4-Aminobutyrate Transaminase / metabolism* DNA, Complementary / genetics DNA, Plant / genetics Fruit / genetics Fruit / growth & development Fruit / metabolism* Gas Chromatography-Mass Spectrometry Gene Expression Genome, Plant Glutamate Decarboxylase / metabolism* Lycopersicon esculentum / genetics Lycopersicon esculentum / growth & development Lycopersicon esculentum / metabolism* RNA, Messenger / analysis Reverse Transcriptase Polymerase Chain Reaction Time Factors gamma-Aminobutyric Acid / metabolism*
IF 4.059
Times Cited 67
WOS Category PLANT SCIENCES CELL BIOLOGY
Resource
Tomato TOMJPF00001