Reference - Detail
|Author||Takada H, Kawana T, Ito Y, Kikuno RF, Mamada H, Araki T, Koga H, Asashima M, Taira M.|
|Title||The RNA-binding protein Mex3b has a fine-tuning system for mRNA regulation in early Xenopus development.|
Post-transcriptional control by RNA-binding proteins is a precise way to assure appropriate levels of gene expression. Here, we identify a novel mRNA regulatory system involving Mex3b (RKHD3) and demonstrate its role in FGF signaling. mex3b mRNA has a 3' long conserved UTR, named 3'LCU, which contains multiple elements for both mRNA destabilization and translational enhancement. Notably, Mex3b promotes destabilization of its own mRNA by binding to the 3'LCU, thereby forming a negative autoregulatory loop. The combination of positive regulation and negative autoregulation constitutes a fine-tuning system for post-transcriptional control. In early embryogenesis, Mex3b is involved in anteroposterior patterning of the neural plate. Consistent with this, Mex3b can attenuate FGF signaling and destabilize mRNAs for the FGF signaling components Syndecan 2 and Ets1b through their 3' UTRs. These data suggest that the 3'LCU-mediated fine-tuning system determines the appropriate level of mex3b expression, which in turn contributes to neural patterning through regulating FGF signaling.
|MeSH||3' Untranslated Regions Amino Acid Sequence Animals Animals, Genetically Modified Base Sequence Body Patterning DNA Primers / genetics Fibroblast Growth Factors / genetics Fibroblast Growth Factors / metabolism Gene Expression Regulation, Developmental Models, Biological Molecular Sequence Data Neural Plate / embryology Neural Plate / metabolism Proto-Oncogene Protein c-ets-1 / genetics Proto-Oncogene Protein c-ets-1 / metabolism RNA Processing, Post-Transcriptional RNA, Messenger / genetics RNA, Messenger / metabolism RNA-Binding Proteins / antagonists & inhibitors RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Sequence Homology, Amino Acid Signal Transduction Syndecan-2 / genetics Syndecan-2 / metabolism Xenopus / embryology* Xenopus / genetics Xenopus / metabolism* Xenopus Proteins / antagonists & inhibitors Xenopus Proteins / genetics Xenopus Proteins / metabolism*|
|WOS Category||DEVELOPMENTAL BIOLOGY|