RRC ID 47921
著者 Younus F, Fraser NJ, Coppin CW, Liu JW, Correy GJ, Chertemps T, Pandey G, Maïbèche M, Jackson CJ, Oakeshott JG.
タイトル Molecular basis for the behavioral effects of the odorant degrading enzyme Esterase 6 in Drosophila.
ジャーナル Sci Rep
Abstract Previous electrophysiological and behavioural studies implicate esterase 6 in the processing of the pheromone cis-vaccenyl acetate and various food odorants that affect aggregation and reproductive behaviours. Here we show esterase 6 has relatively high activity against many of the short-mid chain food esters, but negligible activity against cis-vaccenyl acetate. The crystal structure of esterase 6 confirms its substrate-binding site can accommodate many short-mid chain food esters but not cis-vaccenyl acetate. Immunohistochemical assays show esterase 6 is expressed in non-neuronal cells in the third antennal segment that could be accessory or epidermal cells surrounding numerous olfactory sensilla, including basiconics involved in food odorant detection. Esterase 6 is also produced in trichoid sensilla, but not in the same cell types as the cis-vaccenyl acetate binding protein LUSH. Our data support a model in which esterase 6 acts as a direct odorant degrading enzyme for many bioactive food esters, but not cis-vaccenyl acetate.
巻・号 7
ページ 46188
公開日 2017-4-10
DOI 10.1038/srep46188
PII srep46188
PMID 28393888
PMC PMC5385555
MeSH Animals Arthropod Antennae / enzymology Behavior, Animal / physiology* Carboxylesterase / chemistry Carboxylesterase / metabolism* Catalytic Domain Drosophila Proteins / chemistry Drosophila Proteins / metabolism* Drosophila melanogaster / enzymology* Drosophila melanogaster / physiology* Kinetics Models, Molecular Odorants* Receptors, Odorant / metabolism Structural Homology, Protein Substrate Specificity
IF 3.998
引用数 19
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ショウジョウバエ 6917R-2 6917R-3