Reference - Detail
| RRC ID | 47921 |
|---|---|
| Author | Younus F, Fraser NJ, Coppin CW, Liu JW, Correy GJ, Chertemps T, Pandey G, Maïbèche M, Jackson CJ, Oakeshott JG. |
| Title | Molecular basis for the behavioral effects of the odorant degrading enzyme Esterase 6 in Drosophila. |
| Journal | Sci Rep |
| Abstract |
Previous electrophysiological and behavioural studies implicate esterase 6 in the processing of the pheromone cis-vaccenyl acetate and various food odorants that affect aggregation and reproductive behaviours. Here we show esterase 6 has relatively high activity against many of the short-mid chain food esters, but negligible activity against cis-vaccenyl acetate. The crystal structure of esterase 6 confirms its substrate-binding site can accommodate many short-mid chain food esters but not cis-vaccenyl acetate. Immunohistochemical assays show esterase 6 is expressed in non-neuronal cells in the third antennal segment that could be accessory or epidermal cells surrounding numerous olfactory sensilla, including basiconics involved in food odorant detection. Esterase 6 is also produced in trichoid sensilla, but not in the same cell types as the cis-vaccenyl acetate binding protein LUSH. Our data support a model in which esterase 6 acts as a direct odorant degrading enzyme for many bioactive food esters, but not cis-vaccenyl acetate. |
| Volume | 7 |
| Pages | 46188 |
| Published | 2017-4-10 |
| DOI | 10.1038/srep46188 |
| PII | srep46188 |
| PMID | 28393888 |
| PMC | PMC5385555 |
| MeSH | Animals Arthropod Antennae / enzymology Behavior, Animal / physiology* Carboxylesterase / chemistry Carboxylesterase / metabolism* Catalytic Domain Drosophila Proteins / chemistry Drosophila Proteins / metabolism* Drosophila melanogaster / enzymology* Drosophila melanogaster / physiology* Kinetics Models, Molecular Odorants* Receptors, Odorant / metabolism Structural Homology, Protein Substrate Specificity |
| IF | 3.998 |
| Times Cited | 19 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 9 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | 6917R-2 6917R-3 |