| RRC ID |
47921
|
| Author |
Younus F, Fraser NJ, Coppin CW, Liu JW, Correy GJ, Chertemps T, Pandey G, Maïbèche M, Jackson CJ, Oakeshott JG.
|
| Title |
Molecular basis for the behavioral effects of the odorant degrading enzyme Esterase 6 in Drosophila.
|
| Journal |
Sci Rep
|
| Abstract |
Previous electrophysiological and behavioural studies implicate esterase 6 in the processing of the pheromone cis-vaccenyl acetate and various food odorants that affect aggregation and reproductive behaviours. Here we show esterase 6 has relatively high activity against many of the short-mid chain food esters, but negligible activity against cis-vaccenyl acetate. The crystal structure of esterase 6 confirms its substrate-binding site can accommodate many short-mid chain food esters but not cis-vaccenyl acetate. Immunohistochemical assays show esterase 6 is expressed in non-neuronal cells in the third antennal segment that could be accessory or epidermal cells surrounding numerous olfactory sensilla, including basiconics involved in food odorant detection. Esterase 6 is also produced in trichoid sensilla, but not in the same cell types as the cis-vaccenyl acetate binding protein LUSH. Our data support a model in which esterase 6 acts as a direct odorant degrading enzyme for many bioactive food esters, but not cis-vaccenyl acetate.
|
| Volume |
7
|
| Pages |
46188
|
| Published |
2017-4-10
|
| DOI |
10.1038/srep46188
|
| PII |
srep46188
|
| PMID |
28393888
|
| PMC |
PMC5385555
|
| MeSH |
Animals
Arthropod Antennae / enzymology
Behavior, Animal / physiology*
Carboxylesterase / chemistry
Carboxylesterase / metabolism*
Catalytic Domain
Drosophila Proteins / chemistry
Drosophila Proteins / metabolism*
Drosophila melanogaster / enzymology*
Drosophila melanogaster / physiology*
Kinetics
Models, Molecular
Odorants*
Receptors, Odorant / metabolism
Structural Homology, Protein
Substrate Specificity
|
| IF |
3.998
|
| Times Cited |
19
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Drosophila |
6917R-2
6917R-3 |
