RRC ID 47930
Author Kimura T, Takehana Y, Naruse K.
Title pnp4a Is the Causal Gene of the Medaka Iridophore Mutant guanineless.
Journal G3 (Bethesda)
Abstract See-through medaka lines are suitable for observing internal organs throughout life. They were bred by crossing multiple color mutants. However, some of the causal genes for these mutants have not been identified. The medaka has four pigment cell types: black melanophores, yellow xanthophores, white leucophores, and silvery iridophores. The causal genes of melanophore, xanthophore, and leucophore mutants have been elucidated, but the causal gene for the iridophore mutant remains unknown. Here, we describe the iridophore mutant, guanineless (gu), which exhibits a strong reduction in visible iridophores throughout its larval to adult stages. The gu locus was previously mapped to chromosome 5, but was located near the telomeric region, making it difficult to integrate into the chromosome. We sought the causal gene of gu using synteny analysis with the zebrafish genome and found a strong candidate, purine nucleoside phosphorylase 4a (pnp4a). Gene targeting and complementation testing showed that pnp4a is the causal gene of gu This result will allow the establishment of inbred medaka strains or other useful strains with see-through phenotypes without major disruption in the genetic background of each strain.
Volume 7(4)
Pages 1357-1363
Published 2017-4-3
DOI 10.1534/g3.117.040675
PII g3.117.040675
PMID 28258112
IF 2.781
Times Cited 7
Medaka T5 (MT827) gu (MT58 ) d-rR/TOKYO (MT837) Full length cDNA (olec1e13)