Reference - Detail
| RRC ID | 4795 |
|---|---|
| Author | Oka T, Miyahara M, Yamamoto J, Mitsui N, Fujii T, Tooi O, Kashiwagi K, Takase M, Kashiwagi A, Iguchi T. |
| Title | Application of metamorphosis assay to a native Japanese amphibian species, Rana rugosa, for assessing effects of thyroid system affecting chemicals. |
| Journal | Ecotoxicol Environ Saf |
| Abstract |
The aims of this study were to assess the utility of a metamorphosis assay for detecting thyroid hormone-disrupting chemicals using Rana rugosa, a domestic frog species in Japan, and to compare species differences in sensitivity to thyroxine (T(4)) and propylthiouracil (PTU) among R. rugosa, Xenopus laevis and Xenopus (Silurana) tropicalis. Tadpoles of R. rugosa (TK stages III/IV) were exposed to standard test chemicals for acceleration (T(4)) and inhibition (PTU) of metamorphosis for 28 days in semi-static condition and total body length and developmental stage (TK stage) were recorded every week. T(4) (0.61 and 2.24 microg/L in actual concentrations) and PTU (19.73, 76.83, and 155.67 mg/L in actual concentrations) induced significant acceleration and inhibition of metamorphosis, respectively. The present results indicate that the metamorphosis assay is successfully applied to the domestic frog species, R. rugosa, suggesting this assay can be used for the assessment of chemicals on ecological impacts in wild frog species. |
| Volume | 72(5) |
| Pages | 1400-5 |
| Published | 2009-7-1 |
| DOI | 10.1016/j.ecoenv.2009.03.012 |
| PII | S0147-6513(09)00054-2 |
| PMID | 19394694 |
| MeSH | Animals Antithyroid Agents / toxicity* Biological Assay* Dose-Response Relationship, Drug Endocrine Disruptors / toxicity* Feasibility Studies Female Larva / drug effects Male Metamorphosis, Biological / drug effects* Propylthiouracil / toxicity Ranidae / embryology Ranidae / growth & development* Species Specificity Temperature Thyroid Gland / drug effects* Thyroxine / toxicity Time Factors Tissue Culture Techniques Toxicity Tests / methods* Xenopus laevis / growth & development |
| IF | 4.872 |
| Times Cited | 16 |
| WOS Category | TOXICOLOGY ENVIRONMENTAL SCIENCES |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Policy sources |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Clawed frogs / Newts | |