RRC ID 47988
Author Ueno T, Suzuki H, Hiraishi M, Amano H, Fukuyama H, Sugimoto N.
Title In vivo Magnetic Resonance Microscopy and Hypothermic Anaesthesia of a Disease Model in Medaka.
Journal Sci Rep
Abstract In medical and pharmacological research, various human disease models in small fish, such as medaka (Oryzias latipes), have been created. To investigate these disease models noninvasively, magnetic resonance imaging (MRI) is suitable because these small fish are no longer transparent as adults. However, their small body size requires a high spatial resolution, and a water pool should be avoided to maximize the strength of MRI. We developed in vivo magnetic resonance microscopy (MR microscopy) without a water pool by combining hypothermic anaesthesia and a 14.1 T MR microscope. Using in vivo MR microscopy, we noninvasively evaluated the hepatic steatosis level of a non-alcoholic fatty liver disease model in medaka and followed the individual disease progression. The steatosis level was quantified by the MRI-estimated proton density fat-fraction (MRI-PDFF), which estimates the triglyceride fat concentration in liver tissue and is recognized as an imaging biomarker. The MRI-PDFF results agreed with a histological analysis. Moreover, we optimized the hypothermic anaesthesia procedure to obtain a recovery proportion of 1 in the experiment involving MR microscopy. Recovered medaka could not be distinguished from naïve medaka after the experiment. Therefore, the in vivo MR microscopy will expand the possibilities of a human disease model in fish.
Volume 6
Pages 27188
Published 2016-6-2
DOI 10.1038/srep27188
PII srep27188
PMID 27251889
PMC PMC4890013
MeSH Animals Body Size Cryoanesthesia / methods* Diet, High-Fat Disease Models, Animal Disease Progression Humans Liver / diagnostic imaging* Liver / metabolism Magnetic Resonance Imaging / instrumentation* Magnetic Resonance Imaging / methods Non-alcoholic Fatty Liver Disease / chemically induced Non-alcoholic Fatty Liver Disease / diagnostic imaging* Oryzias Triglycerides / metabolism
IF 4.011
Times Cited 3
Medaka MT894 p53(E241x) MT117 STIII