Reference - Detail
|Author||Shen J, Yokota S, Yokoi H, Suzuki T.|
|Title||Diethylnitrosamine-induced expression of germline-specific genes and pluripotency factors, including vasa and oct4, in medaka somatic cells.|
|Journal||Biochem Biophys Res Commun|
Various methods have been developed to reprogram mammalian somatic cells into pluripotent cells as well as to directly reprogram somatic cells into other cell lineages. We are interested in applying these methods to fish, and here, we examined whether mRNA expression of germline-specific genes (vasa, nanos2, -3) and pluripotency factors (oct4, sox2, c-myc, nanog) is inducible in somatic cells of Japanese medaka (Oryzias latipes). We found that the expression of vasa is induced in the gut and regenerating fin by exposure to a carcinogen, diethylnitrosamine (DEN). Induction of vasa in the gut started on the 5th day of treatment with >50 ppm DEN. In addition, nanos2, -3, oct4, sox2, klf4, c-myc, and nanog were also expressed simultaneously in some vasa-positive gut and regenerating fin samples. Vasa-positive cells were detected by immunohistochemistry (IHC) in the muscle surrounding the gut and in the wound epidermis, blastema, and fibroblast-like cells in regenerating fin. In vasa:GFP transgenic medaka, green fluorescent protein (GFP) fluorescence appeared in the wound epidermis and fibroblast-like cells in the regenerating fin following DEN exposure, in agreement with the IHC data. Our data show that mRNA expression of genes relevant to germ cell specification and pluripotency can be induced in fish somatic cells by exposure to DEN, suggesting the possibility of efficient and rapid cell reprogramming of fish somatic cells.
|MeSH||Animal Fins / cytology Animal Fins / drug effects Animal Fins / metabolism Animals Animals, Genetically Modified Biomarkers / metabolism Carcinogens / pharmacology* Cellular Reprogramming* DEAD-box RNA Helicases / genetics DEAD-box RNA Helicases / metabolism Diethylnitrosamine / pharmacology* Fibroblasts / cytology Fibroblasts / drug effects* Fibroblasts / metabolism Fish Proteins / genetics* Fish Proteins / metabolism Gene Expression Intestinal Mucosa / metabolism Intestines / cytology Intestines / drug effects Muscle, Striated / cytology Muscle, Striated / drug effects Muscle, Striated / metabolism Nanog Homeobox Protein / genetics Nanog Homeobox Protein / metabolism Octamer Transcription Factor-3 / genetics Octamer Transcription Factor-3 / metabolism Oryzias Pluripotent Stem Cells / cytology Pluripotent Stem Cells / drug effects* Pluripotent Stem Cells / metabolism Proto-Oncogene Proteins c-myc / genetics Proto-Oncogene Proteins c-myc / metabolism RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism SOXB1 Transcription Factors / genetics SOXB1 Transcription Factors / metabolism Zinc Fingers|
|WOS Category||BIOCHEMISTRY & MOLECULAR BIOLOGY BIOPHYSICS|