論文 - 詳細
| RRC ID | 47998 |
|---|---|
| 著者 | Ishikawa T, Toyama T, Nakamura Y, Tamada K, Shimizu H, Ninagawa S, Okada T, Kamei Y, Ishikawa-Fujiwara T, Todo T, Aoyama E, Takigawa M, Harada A, Mori K. |
| タイトル | UPR transducer BBF2H7 allows export of type II collagen in a cargo- and developmental stage-specific manner. |
| ジャーナル | J Cell Biol |
| Abstract |
The unfolded protein response (UPR) handles unfolded/misfolded proteins accumulated in the endoplasmic reticulum (ER). However, it is unclear how vertebrates correctly use the total of ten UPR transducers. We have found that ER stress occurs physiologically during early embryonic development in medaka fish and that the smooth alignment of notochord cells requires ATF6 as a UPR transducer, which induces ER chaperones for folding of type VIII (short-chain) collagen. After secretion of hedgehog for tissue patterning, notochord cells differentiate into sheath cells, which synthesize type II collagen. In this study, we show that this vacuolization step requires both ATF6 and BBF2H7 as UPR transducers and that BBF2H7 regulates a complete set of genes (Sec23/24/13/31, Tango1, Sedlin, and KLHL12) essential for the enlargement of COPII vesicles to accommodate long-chain collagen for export, leading to the formation of the perinotochordal basement membrane. Thus, the most appropriate UPR transducer is activated to cope with the differing physiological ER stresses of different content types depending on developmental stage. |
| 巻・号 | 216(6) |
| ページ | 1761-1774 |
| 公開日 | 2017-6-5 |
| DOI | 10.1083/jcb.201609100 |
| PII | jcb.201609100 |
| PMID | 28500182 |
| PMC | PMC5461018 |
| MeSH | Activating Transcription Factor 6 / genetics Activating Transcription Factor 6 / metabolism Animals Animals, Genetically Modified Basement Membrane / metabolism Basic-Leucine Zipper Transcription Factors / genetics Basic-Leucine Zipper Transcription Factors / metabolism* COP-Coated Vesicles / metabolism* Collagen Type II / metabolism* Embryo, Nonmammalian / metabolism Endoplasmic Reticulum / metabolism Endoplasmic Reticulum Stress Fish Proteins / genetics Fish Proteins / metabolism* Gene Expression Regulation, Developmental Genotype HCT116 Cells Humans Notochord / metabolism* Oryzias / embryology Oryzias / genetics Oryzias / metabolism* Phenotype Protein Transport Time Factors Transcription, Genetic Transfection Unfolded Protein Response* Vacuoles / metabolism |
| IF | 8.811 |
| 引用数 | 21 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 47 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| メダカ | PBiP-EGFP (TG998) PBrachyury-Venus (TG1005) OK-Cab (MT830) Bac clone (GOLWFno460_f02 GOLWFno430_o13) |