論文 - 詳細
| RRC ID | 48087 |
|---|---|
| 著者 | Uezumi A, Nakatani M, Ikemoto-Uezumi M, Yamamoto N, Morita M, Yamaguchi A, Yamada H, Kasai T, Masuda S, Narita A, Miyagoe-Suzuki Y, Takeda S, Fukada S, Nishino I, Tsuchida K. |
| タイトル | Cell-Surface Protein Profiling Identifies Distinctive Markers of Progenitor Cells in Human Skeletal Muscle. |
| ジャーナル | Stem Cell Reports |
| Abstract |
Skeletal muscle contains two distinct stem/progenitor populations. One is the satellite cell, which acts as a muscle stem cell, and the other is the mesenchymal progenitor, which contributes to muscle pathogeneses such as fat infiltration and fibrosis. Detailed and accurate characterization of these progenitors in humans remains elusive. Here, we performed comprehensive cell-surface protein profiling of the two progenitor populations residing in human skeletal muscle and identified three previously unrecognized markers: CD82 and CD318 for satellite cells and CD201 for mesenchymal progenitors. These markers distinguish myogenic and mesenchymal progenitors, and enable efficient isolation of the two types of progenitors. Functional study revealed that CD82 ensures expansion and preservation of myogenic progenitors by suppressing excessive differentiation, and CD201 signaling favors adipogenesis of mesenchymal progenitors. Thus, cell-surface proteins identified here are not only useful markers but also functionally important molecules, and provide valuable insight into human muscle biology and diseases. |
| 巻・号 | 7(2) |
| ページ | 263-78 |
| 公開日 | 2016-8-9 |
| DOI | 10.1016/j.stemcr.2016.07.004 |
| PII | S2213-6711(16)30128-X |
| PMID | 27509136 |
| PMC | PMC4983081 |
| MeSH | Adipogenesis Antibodies / metabolism Antigens, CD / metabolism Biomarkers Cell Membrane / metabolism* Cell Separation Humans Membrane Proteins / metabolism* Muscle, Skeletal / cytology* Proteomics / methods* Stem Cells / metabolism* |
| IF | 6.032 |
| 引用数 | 39 |
| WOS 分野 | CELL & TISSUE ENGINEERING CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 23 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 遺伝子材料 | pCAG-HIVgp (RDB04394) |