論文 - 詳細
| RRC ID | 48097 |
|---|---|
| 著者 | Fukushima D, Kitamura N, Nakanishi S. |
| タイトル | Nucleotide sequence of cloned cDNA for human pancreatic kallikrein. |
| ジャーナル | Biochemistry |
| Abstract |
Cloned cDNA sequences for human pancreatic kallikrein have been isolated and determined by molecular cloning and sequence analysis. The identity between human pancreatic and urinary kallikreins is indicated by the complete coincidence between the amino acid sequence deduced from the cloned cDNA sequence and that reported partially for urinary kallikrein. The active enzyme form of the human pancreatic kallikrein consists of 238 amino acids and is preceded by a signal peptide and a profragment of 24 amino acids. A sequence comparison of this with other mammalian kallikreins indicates that key amino acid residues required for both serine protease activity and kallikrein-like cleavage specificity are retained in the human sequence, and residues corresponding to some external loops of the kallikrein diverge from other kallikreins. Analyses by RNA blot hybridization, primer extension, and S1 nuclease mapping indicate that the pancreatic kallikrein mRNA is also expressed in the kidney and sublingual gland, suggesting the active synthesis of urinary kallikrein in these tissues. Furthermore, the tissue-specific regulation of the expression of the members of the human kallikrein gene family has been discussed. |
| 巻・号 | 24(27) |
| ページ | 8037-43 |
| 公開日 | 1985-12-31 |
| DOI | 10.1021/bi00348a030 |
| PMID | 3004571 |
| MeSH | Amino Acid Sequence Base Sequence Cloning, Molecular* DNA / isolation & purification DNA / metabolism* DNA Restriction Enzymes Humans Kallikreins / genetics* Pancreas / enzymology* |
| IF | 2.865 |
| 引用数 | 154 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 25 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | phKK - 25 (RDB13180) |