| RRC ID |
48110
|
| Author |
Kitano J, Yamazaki Y, Kimura K, Masukado T, Nakajima Y, Nakanishi S.
|
| Title |
Tamalin is a scaffold protein that interacts with multiple neuronal proteins in distinct modes of protein-protein association.
|
| Journal |
J Biol Chem
|
| Abstract |
Tamalin is a scaffold protein that comprises multiple protein-interacting domains, including a 95-kDa postsynaptic density protein (PSD-95)/discs-large/ZO-1 (PDZ) domain, a leucine-zipper region, and a carboxyl-terminal PDZ binding motif. Tamalin forms a complex with metabotropic glutamate receptors and guanine nucleotide exchange factor cytohesins and promotes intracellular trafficking and cell surface expression of group 1 metabotropic glutamate receptors. In the present study, using several different approaches we have shown that tamalin interacts with multiple neuronal proteins through its distinct protein-binding domains. The PDZ domain of tamalin binds to the PDZ binding motifs of SAP90/PSD-95-associated protein and tamalin itself, whereas the PDZ binding motif of tamalin is capable of interacting with the PDZ domain of S-SCAM. In addition, tamalin forms a complex with PSD-95 and Mint2/X11beta/X11L by mechanisms different from the PDZ-mediated interaction. Tamalin has the ability to assemble with these proteins in vivo; their protein complex with tamalin was verified by coimmunoprecipitation of rat brain lysates. Interestingly, the distinct protein-interacting domains of tamalin are evolutionarily conserved, and mRNA expression is developmentally up-regulated at the postnatal period. The results indicate that tamalin exists as a key element that forms a protein complex with multiple postsynaptic and protein-trafficking scaffold proteins.
|
| Volume |
278(17)
|
| Pages |
14762-8
|
| Published |
2003-4-25
|
| DOI |
10.1074/jbc.M300184200
|
| PII |
S0021-9258(19)30269-8
|
| PMID |
12586822
|
| MeSH |
Adaptor Proteins, Signal Transducing
Amino Acid Sequence
Animals
Cadherins*
Carrier Proteins / chemistry
Carrier Proteins / metabolism*
Carrier Proteins / physiology
Conserved Sequence
Disks Large Homolog 4 Protein
Guanylate Kinases
Intracellular Signaling Peptides and Proteins
Membrane Proteins
Nerve Tissue Proteins / chemistry
Nerve Tissue Proteins / metabolism*
Neurons / chemistry
Protein Binding
Protein Structure, Tertiary
Rats
SAP90-PSD95 Associated Proteins
Sequence Alignment
|
| IF |
4.238
|
| Times Cited |
53
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
Tamalin pcDNA3.1 (RDB13213) |
