論文 - 詳細
| RRC ID | 48110 |
|---|---|
| 著者 | Kitano J, Yamazaki Y, Kimura K, Masukado T, Nakajima Y, Nakanishi S. |
| タイトル | Tamalin is a scaffold protein that interacts with multiple neuronal proteins in distinct modes of protein-protein association. |
| ジャーナル | J Biol Chem |
| Abstract |
Tamalin is a scaffold protein that comprises multiple protein-interacting domains, including a 95-kDa postsynaptic density protein (PSD-95)/discs-large/ZO-1 (PDZ) domain, a leucine-zipper region, and a carboxyl-terminal PDZ binding motif. Tamalin forms a complex with metabotropic glutamate receptors and guanine nucleotide exchange factor cytohesins and promotes intracellular trafficking and cell surface expression of group 1 metabotropic glutamate receptors. In the present study, using several different approaches we have shown that tamalin interacts with multiple neuronal proteins through its distinct protein-binding domains. The PDZ domain of tamalin binds to the PDZ binding motifs of SAP90/PSD-95-associated protein and tamalin itself, whereas the PDZ binding motif of tamalin is capable of interacting with the PDZ domain of S-SCAM. In addition, tamalin forms a complex with PSD-95 and Mint2/X11beta/X11L by mechanisms different from the PDZ-mediated interaction. Tamalin has the ability to assemble with these proteins in vivo; their protein complex with tamalin was verified by coimmunoprecipitation of rat brain lysates. Interestingly, the distinct protein-interacting domains of tamalin are evolutionarily conserved, and mRNA expression is developmentally up-regulated at the postnatal period. The results indicate that tamalin exists as a key element that forms a protein complex with multiple postsynaptic and protein-trafficking scaffold proteins. |
| 巻・号 | 278(17) |
| ページ | 14762-8 |
| 公開日 | 2003-4-25 |
| DOI | 10.1074/jbc.M300184200 |
| PII | S0021-9258(19)30269-8 |
| PMID | 12586822 |
| MeSH | Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals Cadherins* Carrier Proteins / chemistry Carrier Proteins / metabolism* Carrier Proteins / physiology Conserved Sequence Disks Large Homolog 4 Protein Guanylate Kinases Intracellular Signaling Peptides and Proteins Membrane Proteins Nerve Tissue Proteins / chemistry Nerve Tissue Proteins / metabolism* Neurons / chemistry Protein Binding Protein Structure, Tertiary Rats SAP90-PSD95 Associated Proteins Sequence Alignment |
| IF | 4.238 |
| 引用数 | 53 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | Tamalin pcDNA3.1 (RDB13213) |