| RRC ID |
48111
|
| Author |
Kishikawa S, Murata T, Kimura H, Shiota K, Yokoyama KK.
|
| Title |
Regulation of transcription of the Dnmt1 gene by Sp1 and Sp3 zinc finger proteins.
|
| Journal |
Eur J Biochem
|
| Abstract |
The Sp family is a family of transcription factors that bind to cis-elements in the promoter regions of various genes. Regulation of transcription by Sp proteins is based on interactions between a GC-rich binding site (GGGCGG) in DNA and C-terminal zinc finger motifs in the proteins. In this study, we characterized the GC-rich promoter of the gene for the DNA methyltransferase (Dnmt1) that is responsible for methylation of cytosine residues in mammals and plays a role in gene silencing. We found that a cis-element (nucleotides -161 to -147) was essential for the expression of the mouse gene for Dnmt1. DNA-binding assays indicated that transcription factors Sp1 and Sp3 bound to the same cis-element in this region in a dose-dependent manner. In Drosophila SL2 cells, which lack the Sp family of transcription factors, forced expression of Sp1 or Sp3 enhanced transcription from the Dnmt1 promoter. Stimulation by Sp1 and Sp3 were independent phenomena. Furthermore, cotransfection reporter assays with a p300-expression plasmid revealed the activation of the promoter of the Dnmt1 gene in the presence of Sp3. The transcriptional coactivator p300 interacted with Sp3 in vivo and in vitro. Our results indicate that expression of the Dnmt1 gene is controled by Sp1 and Sp3 and that p300 is involved in the activation by Sp3.
|
| Volume |
269(12)
|
| Pages |
2961-70
|
| Published |
2002-6-1
|
| DOI |
10.1046/j.1432-1033.2002.02972.x
|
| PII |
2972
|
| PMID |
12071960
|
| MeSH |
3T3 Cells
Adenine / metabolism
Animals
Binding Sites
Cells, Cultured
DNA (Cytosine-5-)-Methyltransferase 1
DNA (Cytosine-5-)-Methyltransferases / genetics*
DNA (Cytosine-5-)-Methyltransferases / metabolism
DNA-Binding Proteins / metabolism
DNA-Binding Proteins / physiology*
Drosophila melanogaster
E1A-Associated p300 Protein
Genes, Regulator / genetics*
Guanosine / metabolism
Mice
Nuclear Proteins / metabolism
Nuclear Proteins / physiology
Oligodeoxyribonucleotides / metabolism
Promoter Regions, Genetic
Sp1 Transcription Factor / metabolism
Sp1 Transcription Factor / physiology*
Sp3 Transcription Factor
Trans-Activators / metabolism
Trans-Activators / physiology
Transcription Factors / metabolism
Transcription Factors / physiology*
Transcriptional Activation / physiology*
Zinc Fingers / physiology
|
| Times Cited |
73
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
D1 (RDB3179)
D2 (RDB3180)
D4 (RDB3181). |
| Human and Animal Cells |
S2(RCB1153) |
