RRC ID 48152
Author Lee JJ, Joo ES, Kim EB, Jeon SH, Srinivasan S, Jung HY, Kim MK.
Title Hymenobacter rubidus sp. nov., bacterium isolated from a soil.
Journal Antonie Van Leeuwenhoek
Abstract Strain DG7B(T) was isolated from a soil sample collected in Seoul, Republic of Korea and was observed to be a gram-negative, short-rod shaped and non-motile bacterium. Its 16S rRNA gene sequence is closely related to those of Hymenobacter terrae DG7A(T) (97.8 % similarity), H. soli PB17(T) (97.5 %), H. glaciei VUG-A130(T) (96.4 %), H. saemangeumensis GSR0100(T) (95.7 %), H. ruber PB156(T) (95.3 %), and H. antarcticus VUG-A42aa(T) (95.3 %). The low levels of DNA-DNA relatedness (<50.3 %) with the above species identified strain DG7B(T) as a novel species in the genus Hymenobacter. The genomic DNA G+C content was determined to be 54.9 %. Growth of strain DG7B(T) was observed at 12-30 °C (optimum at 25 °C) and pH 6.0-11.0 (optimum at pH 7). The cells tolerate <0.5 % NaCl. A UV-visible scan of an ethanol extract of the whole cell pigment showed absorbance peaks at 264.5, 320.0, and 481.5 nm, so the pigment type was determined to be 2'-hydroxyflexixanthin. Chemotaxonomic data showed that strain DG7B(T) possesses menaquinone-7 as the predominant isoprenoid quinone, sym-homospermidine as the major polyamine, phosphatidylethanolamine as the predominant polar lipid and iso-C15:0, anteiso-C15:0 and summed feature 3 (C16:1 ω7c/C16:1 ω7c) as the major fatty acids. Strain DG7B(T) showed low-level resistance to ultraviolet C. Based on the polyphasic analysis, it is concluded that strain DG7B(T) (=KCTC 32553(T) = KEMB 9004-166(T) = JCM 30008(T)) should be classified as the type strain of a novel Hymenobacter species, for which the name Hymenobacter rubidus sp. nov. is proposed.
Volume 109(3)
Pages 457-66
Published 2016-3
DOI 10.1007/s10482-016-0652-2
PII 10.1007/s10482-016-0652-2
PMID 26809281
MeSH Bacterial Typing Techniques Base Composition Chlamydiaceae / classification* Chlamydiaceae / genetics Chlamydiaceae / isolation & purification Chlamydiaceae / radiation effects Genome, Bacterial Microbial Viability / radiation effects Phenotype Phylogeny RNA, Ribosomal, 16S / genetics Radiation Tolerance / radiation effects Sequence Analysis, DNA Soil Microbiology* Ultraviolet Rays
IF 1.588
Times Cited 1
WOS Category MICROBIOLOGY
Resource
General Microbes JCM 30008 JCM 17225 JCM 17217