RRC ID |
48165
|
著者 |
Fan J, Liu C, Ma Z, Zhou S, Li W, Li J, Chu L, Wang X, Xiang W.
|
タイトル |
Dactylosporangium solaniradicis sp. nov., a novel actinobacterium isolated from a root of tomato (Solanum lycopersicum L.).
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ジャーナル |
Antonie Van Leeuwenhoek
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Abstract |
A novel actinobacterium, designated strain NEAU-FJL2(T), was isolated from a tomato root (Solanum lycopersicum L.) and characterised using a polyphasic approach. Morphological and chemotaxonomic properties of strain NEAU-FJL2(T) are consistent with the description of the genus Dactylosporangium. Finger-shaped sporangia were observed to form on short sporangiophores branching from the substrate hyphae. The cell wall peptidoglycan was found to contain meso- and 3-hydroxy-diaminopimelic acids; arabinose, mannose, rhamnose and xylose were found as whole-cell sugars. The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones were identified as MK-9(H8) and MK-9(H6). The major fatty acids were identified as iso-C16:0, C16:1 ω7c and iso-C15:0. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strain NEAU-FJL2(T) belongs to the genus Dactylosporangium, with Dactylosporangium sucinum JCM 19831(T) (99.3 % 16S rRNA gene sequence similarity), Dactylosporangium darangshiense JCM 17441(T) (99.2 %), Dactylosporangium fulvum JCM 5631(T) (98.9 %) and Dactylosporangium roseum JCM 3364(T) (98.8 %) as the nearest phylogenetic relatives. However, a combination of DNA-DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-FJL2(T) can be distinguished from them. Consequently, it is proposed that strain NEAU-FJL2(T) represents a novel species of the genus Dactylosporangium, for which the name Dactylosporangium solaniradicis sp. nov. is proposed. The type strain is NEAU-FJL2(T) (=CGMCC 4.7302(T) = DSM 100814(T)).
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巻・号 |
109(7)
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ページ |
971-7
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公開日 |
2016-7-1
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DOI |
10.1007/s10482-016-0697-2
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PII |
10.1007/s10482-016-0697-2
|
PMID |
27091125
|
MeSH |
Cell Wall / chemistry
China
DNA, Bacterial / genetics
DNA, Ribosomal / genetics
Diaminopimelic Acid / metabolism
Fatty Acids / metabolism
Micromonosporaceae / classification*
Micromonosporaceae / genetics
Micromonosporaceae / isolation & purification*
Micromonosporaceae / metabolism
Nucleic Acid Hybridization
Peptidoglycan / metabolism
Phenotype
Phospholipids / metabolism
Phylogeny
Plant Roots / microbiology
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil Microbiology
Solanum lycopersicum / microbiology*
|
IF |
2.674
|
引用数 |
2
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 19831
JCM 17441
JCM 5631
JCM 3364 |